CD2 antibody (Biotin)

Catalog No. ABIN793370

Product Details anti-CD2 Antibody (Biotin)

Target: CD2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Human CD2

Purification: Protein A/G purified

Sterility: Sterile filtered

Immunogen: T Lymphocytes activated by mixed lymphocyte culture.

Clone: T6-3

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo

Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.08 % sodium azide, 0.2 % carrier protein, sterile-filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for CD2

Target: CD2

Alternative Name: CD2 (CD2 Products)

Background: Name/Gene ID: CD2

Synonyms: CD2, CD2 antigen, Erythrocyte receptor, LFA-3 receptor, LFA-2, Lymphocyte-function antigen-2, T11, SRBC, CD2 Molecule, Rosette receptor, T-cell surface antigen CD2

Gene ID: 914

UniProt: P06729