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Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE) antibody (FITC)

Details for Product No. ABIN810081, Supplier: Log in to see
Antigen
  • A530055J10
  • CD103
  • aM290
  • alpha-E1
  • alpha-M290
  • HUMINAE
Reactivity
Human, Non-Human Primate
156
99
27
6
4
3
1
1
Host
Mouse
138
69
42
13
5
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
41
41
21
16
5
5
5
4
3
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
205
81
61
48
44
34
11
9
8
8
7
7
2
2
2
2
1
1
1
Supplier
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Immunogen HTLV-1 induced human T cell line MAPS16
Clone Ber-ACT8
Isotype IgG1
Specificity The mouse monoclonal antibody Ber-ACT8 recognizes CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes. HLDA V, WS Code A067
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Alternative Name ITGAE (ITGAE Antibody Abstract)
Background CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.
Gene ID 3682
Pathways
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 4 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Product cited in: Zhang, Bertucci, Ramsey-Goldman et al.: "Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 10, pp. 6346-58, 2009 (PubMed).

Jaensson, Uronen-Hansson, Pabst et al.: "Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans." in: The Journal of experimental medicine, Vol. 205, Issue 9, pp. 2139-49, 2008 (PubMed).

Kost, Holden, Mann: "Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis." in: Cytometry. Part B, Clinical cytometry, Vol. 74, Issue 5, pp. 282-6, 2008 (PubMed).

Cardoso, Garlet, Moreira et al.: "Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 311-8, 2008 (PubMed).

Sigmundsdottir, Johnston, Gudjonsson et al.: "Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin." in: Experimental dermatology, Vol. 13, Issue 7, pp. 426-34, 2004 (PubMed).

León, Roy: "Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 6, pp. 804-9, 2004 (PubMed).

Poles, Barsoum, Yu et al.: "Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy." in: Journal of virology, Vol. 77, Issue 19, pp. 10456-67, 2003 (PubMed).

Brandes, Willimann, Lang et al.: "Flexible migration program regulates gamma delta T-cell involvement in humoral immunity." in: Blood, Vol. 102, Issue 10, pp. 3693-701, 2003 (PubMed).

French, Cresswell, Wong et al.: "T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?" in: British journal of cancer, Vol. 87, Issue 9, pp. 1034-41, 2002 (PubMed).