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Tripartite Motif Containing 28 (TRIM28) (AA 827-831) antibody

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Synonyms TRIM28, FLA3, KAP-1, KAP-3, KAP3, SMAP, Smg-GDS, dJ190I16.1, KAP1, RNF96, TF1B, TIF1B, TIF1BETA, kap1, rnf96, tf1b, tif1b, AA408787, KRIP-1, Tif1b, Tif1beta, Kap1, Krip1
AA 827-831
(31), (22), (18), (15), (14), (13), (11), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(206), (71), (52), (5), (3), (1), (1), (1), (1), (1)
(127), (51), (32)
(11), (11), (11), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB)
(171), (95), (72), (64), (39), (25), (24), (22), (8), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 100 μL
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Immunogen Peptide sequence around AA 827-831 (L-S-G-G-P) derived from Human TIF1b. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous level of total TIF1b protein.
Purification Affinity purified
Alternative Name TIF1beta (TRIM28 Antibody Abstract)
Background Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.
Molecular Weight 110 kDa
Gene ID 10155
NCBI Accession NP_005753
UniProt Q13263
Pathways Hedgehog Signaling
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Agata, Matsuda, Shimizu: "Two novel Krüppel-associated box-containing zinc-finger proteins, KRAZ1 and KRAZ2, repress transcription through functional interaction with the corepressor KAP-1 (TIF1beta/KRIP-1)." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 16412-22, 1999 (PubMed).

Moosmann, Georgiev, Le Douarin et al.: "Transcriptional repression by RING finger protein TIF1 beta that interacts with the KRAB repressor domain of KOX1." in: Nucleic acids research, Vol. 24, Issue 24, pp. 4859-67, 1997 (PubMed).

Friedman, Fredericks, Jensen et al.: "KAP-1, a novel corepressor for the highly conserved KRAB repression domain." in: Genes & development, Vol. 10, Issue 16, pp. 2067-78, 1996 (PubMed).

Catalog No. ABIN871735
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