Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody

Details for Product No. ABIN184913, Supplier: Log in to see
Antigen
  • KAP1
  • RNF96
  • TF1B
  • TIF1B
  • TRIM28
  • kap1
  • rnf96
  • tf1b
  • tif1b
  • AA408787
  • KAP-1
  • KRIP-1
  • Tif1b
  • Tif1beta
  • Kap1
  • Krip1
  • TIF1BETA
  • tripartite motif containing 28
  • tripartite motif containing 28 L homeolog
  • tripartite motif-containing 28
  • TRIM28
  • trim28.L
  • Trim28
Alternatives
anti-Human Tripartite Motif Containing 28 antibody for Western Blotting
Epitope
C-Term
31
22
17
15
15
12
10
7
6
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
232
107
80
9
9
8
6
5
5
3
1
1
1
1
Host
Goat
147
55
35
Clonality
Polyclonal
Conjugate
Un-conjugated
12
11
10
5
5
4
3
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
193
107
94
66
41
31
30
26
8
7
6
5
5
3
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Purpose KAP1 / TRIM28
Immunogen Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.
Blocking Peptide Blocking peptide for this product available: ABIN369934
Sequence SSQELSGGPG DGP
Predicted Reactivity Human, Mouse
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name TRIM28 (TRIM28 Antibody Abstract)
Background TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein
Gene ID 10155
NCBI Accession NP_005753
Pathways Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
Application Notes

DS IHC Results: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:2000.

Comment

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 (2 µg/mL) staining of HepG2 lysate (35 µg protein in RIPA buffer). Primary...
 image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 (4 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retr...
Immunohistochemistry (IHC) image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 (2µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retri...
Western Blotting (WB) image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabi...
Immunofluorescence (IF) image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabi...
Flow Cytometry (FACS) image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line),...
Product cited in: Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, 2010 (PubMed).

Background publications Friedman, Fredericks, Jensen, Speicher, Huang, Neilson, Rauscher: "KAP-1, a novel corepressor for the highly conserved KRAB repression domain." in: Genes & development, Vol. 10, Issue 16, pp. 2067-78, 1996 (PubMed).

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