For best experience we recommend to activate Javascript in your browser.

AKT 1/2/3 antibody (pSer473) (AbBy Fluor® 350)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Chicken, Fish WB, IF (cc), IF (p) Host: Rabbit Polyclonal AbBy Fluor® 350

Catalog No. ABIN876341

Datasheet as PDF

Target See all AKT 1/2/3 products

AKT 1/2/3
Binding Specificity
22

21

20

20

19

19

16

16

16

16

7

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer473
Reactivity
153

144

129

43

30

25

23

14

7

6

4

4

1
Human, Mouse, Rat, Cow, Rabbit, Chicken, Fish
Host
150

3
Rabbit
Compatible Secondaries
Clonality
139

14
Polyclonal
Conjugate
40

9

7

6

6

6

6

6

6

6

6

6

6

6

6

5

2

2

2

2

2

2

2

2

2

2
This AKT 1/2/3 antibody is conjugated to AbBy Fluor® 350
Application
133

82

78

77

33

32

30

18

9

7

6

3
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity

Chicken, Cow, Fish, Human, Mouse, Rabbit, Rat

Predicted Reactivity

Dog,Cow,Sheep

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473

Isotype

IgG
anti-AKT 1/2/3 (AA 401-479) antibody
Reactivity: Human, Mouse, Rat, Cow, Pig WB, FACS, IF (cc), ELISA, IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 (pSer473) antibody
Reactivity: Human, Mouse, Rat, Cow, Rabbit, Chicken, Fish WB, IF (cc), ELISA, IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 antibody
Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 (pSer472) antibody
Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 antibody
Reactivity: Human, Mouse, Rat, Chimpanzee WB, IHC, FACS, ELISA Host: Mouse Monoclonal 14E5-16C8-25F6 unconjugated

anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody
Reactivity: Human, Mouse, Rat WB, FACS, IF (cc), ELISA, IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 (pTyr474) antibody
Reactivity: Human, Mouse WB, FACS, IF (cc), ELISA, IF (p), IHC (p), IHC (fro), ICC Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody
Reactivity: Human, Mouse, Rat WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibody
Reactivity: Human, Mouse, Rat WB, FACS, IF (cc), ELISA, IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-AKT 1/2/3 (AA 278-312) antibody
Reactivity: Human, Rat WB, IHC (p) Host: Rabbit Polyclonal RB43841 unconjugated

Application Notes

IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date

12 months
Target

AKT 1/2/3

Alternative Name

AKT1/2/3 (AKT 1/2/3 Products)

Background

Synonyms: Akt, RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, RAC-PK-alpha, Akt1
Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Gene ID

24185

UniProt

P47196