CD1a Molecule (CD1A) antibody

Antigen: CD1a Molecule (CD1A)

Synonyms: R4, T6, CD1, FCB6, HTA1, CD1A, CD1.1, CD1.2, CD1A2

Clonality: Monoclonal (HI149)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN94018

Additional Information

Alternative name: CD1a

Gene ID: 1q22-q23

Immunogen: Human thymocytes

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: HI149

Description: CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteinsserve as antigen-presenting molecules for a subset of T cells that responds to specific lipidsand glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens suchas gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a isexcluded from late endosomal compartments and instead traffics independently in therecycling pathway of the early endocytic system, and CD1a antigen presentation isindependent upon vesicular acidification.

Specificity: The antibody HI149 reacts with CD1a (T6), a 49 KDa polypeptide associated withbeta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendriticcells and some T cell leukemias and lymphomas.The antibody does not react with peripheral blood T and B lymphocytes, monocytes,granulocytes, platelets and erythrocytes.HLDA V, WS Code CD01.01

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein G-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Saeemann, Parolini, Boehmig et al.: "Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells." in: Journal of leukocyte biology, Vol. 71, Issue 2, pp. 238-46, 2002 (PubMed).

Hunger, Sieling, Ochoa et al.: "Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells." in: The Journal of clinical investigation, Vol. 113, Issue 5, pp. 701-8, 2004 (PubMed).

McKenna, Asplund, Kroft: "Immunophenotypic analysis of hematogones (B-lymphocyte precursors) and neoplastic lymphoblasts by 4-color flow cytometry." in: Leukemia & lymphoma, Vol. 45, Issue 2, pp. 277-85, 2004 (PubMed).

Demedts, Brusselle, Vermaelen et al.: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005 (PubMed).

Kirsch, Zeyda, Stuhlmeier et al.: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, 2005 (PubMed).

Chen, Murakami, Oppenheim et al.: "Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking." in: Blood, Vol. 106, Issue 7, pp. 2409-16, 2005 (PubMed).

Perros, Dorfmueller, Souza et al.: "Dendritic cell recruitment in lesions of human and experimental pulmonary hypertension." in: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology, Vol. 29, Issue 3, pp. 462-8, 2007 (PubMed).

Angel, Lala, Chen et al.: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Favali, Tavares, Claruencio et al.: "Leishmania amazonensis infection impairs differentiation and function of human dendritic cells." in: Journal of leukocyte biology, Vol. 82, Issue 6, pp. 1401-6, 2007 (PubMed).

Mayer, Irschick, Moser et al.: "Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers." in: Investigative ophthalmology & visual science, Vol. 48, Issue 10, pp. 4459-67, 2007 (PubMed).