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CD1a Molecule (CD1A) antibody (FITC)

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Synonyms CD1A, CD1.1, CD1.2, CD1A2, CD1, FCB6, HTA1, R4, T6, CD1B, AI747460, Cd1a, Cd1d, Ly-38
(317), (17), (14), (14), (8), (6), (4)
(253), (81)
Clonality (Clone)
Monoclonal ()
(35), (33), (18), (11), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS)
(182), (82), (82), (56), (50), (45), (42), (12), (12), (12), (3), (3), (3), (1), (1), (1), (1)
Pubmed 13 references available
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Quantity 100 tests
Shipping to United States ( )
Availability Will be delivered in 4 to 5 Business Days
Immunogen Human thymocytes
Clone HI149
Isotype IgG1
Specificity The antibody HI149 reacts with CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes. HLDA V, WS Code CD01.01
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Alternative Name CD1A (CD1A Antibody Abstract)
Background CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Product cited in: Mayer, Irschick, Moser et al.: "Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers." in: Investigative ophthalmology & visual science, Vol. 48, Issue 10, pp. 4459-67, 2007 (PubMed).

Angel, Lala, Chen et al.: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Perros, Dorfmüller, Souza et al.: "Dendritic cell recruitment in lesions of human and experimental pulmonary hypertension." in: The European respiratory journal, Vol. 29, Issue 3, pp. 462-8, 2007 (PubMed).

Chen, Murakami, Oppenheim et al.: "Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking." in: Blood, Vol. 106, Issue 7, pp. 2409-16, 2005 (PubMed).

Kirsch, Zeyda, Stuhlmeier et al.: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, 2005 (PubMed).

Demedts, Brusselle, Vermaelen et al.: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005 (PubMed).

McKenna, Asplund, Kroft: "Immunophenotypic analysis of hematogones (B-lymphocyte precursors) and neoplastic lymphoblasts by 4-color flow cytometry." in: Leukemia & lymphoma, Vol. 45, Issue 2, pp. 277-85, 2004 (PubMed).

Hunger, Sieling, Ochoa et al.: "Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells." in: The Journal of clinical investigation, Vol. 113, Issue 5, pp. 701-8, 2004 (PubMed).

Säemann, Parolini, Böhmig et al.: "Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells." in: Journal of leukocyte biology, Vol. 71, Issue 2, pp. 238-46, 2002 (PubMed).

Background publications Favali, Tavares, Claruencio et al.: "Leishmania amazonensis infection impairs differentiation and function of human dendritic cells." in: Journal of leukocyte biology, Vol. 82, Issue 6, pp. 1401-6, 2007 (PubMed).

Raftery, Winau, Kaufmann et al.: "CD1 antigen presentation by human dendritic cells as a target for herpes simplex virus immune evasion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 9, pp. 6207-14, 2006 (PubMed).

Hiromatsu, Dascher, Sugita et al.: "Characterization of guinea-pig group 1 CD1 proteins." in: Immunology, Vol. 106, Issue 2, pp. 159-72, 2002 (PubMed).

Sugita, Grant, van Donselaar et al.: "Separate pathways for antigen presentation by CD1 molecules." in: Immunity, Vol. 11, Issue 6, pp. 743-52, 2000 (PubMed).

Catalog No. ABIN94019
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