Keratin 19 (KRT19) antibody

Antigen: Keratin 19 (KRT19)

Synonyms: K19, CK19, K1CS, MGC15366, EndoC, Krt1-19, AI663979, Krt-1.19, MGC25344, Ka19, MGC124534, MGC156553, k19, ck19, k1cs, krt9, krt15, MGC76282, KRT19, GK-19, MGC83069, LOC514812, MGC154386, DKFZp469N0613

Clonality: Monoclonal (BA-17)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN94288

Additional Information

Alternative name: Cytokeratin 19

Immunogen: Mammary organoids.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: BA-17

Description: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity: The antibody BA-17 reacts with cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 isnot expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix

Restrictions: For Research Use only

Images

Detection of cytokeratin 19 in MCF-7 cell lysate by mouse monoclonal antibody BA-17

Publications

Publications: Bartek, Taylor-Papadimitriou, Miller et al.: "Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 299-306, 1985 (PubMed).

Bartek, Durban, Hallowes et al.: "A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins." in: Journal of cell science, Vol. 75, pp. 17-33, 1985 (PubMed).

Lewis, Klimstra, Varmus: "The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer." in: Genes & development, Vol. 17, Issue 24, pp. 3127-38, 2003 (PubMed).