Keratin 19 (KRT19) antibody

Details for Product No. ABIN94290
Antigen
Reactivity
Human
721
206
119
12
11
10
9
6
5
4
3
2
2
2
2
2
1
1
1
1
1
Host
Mouse
506
223
13
5
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
52
22
15
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
5
5
5
5
4
3
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
435
301
242
224
217
214
114
68
52
48
24
15
14
12
3
2
2
2
1
1
Options
Immunogen MCF-7 human breast adenocarcinoma cell line
Clone A53-B-A2
Isotype IgG2a
Specificity The antibody A53-B/A2 reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Cytokeratin 19 (KRT19 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Keratin 19 (KRT19) antibody (ABIN94290) Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human b...
Product cited in: Kasper, Moll, Stosiek, Karsten: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, 1988 (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, 1985 (PubMed).