Denticleless E3 Ubiquitin Protein Ligase Homolog (Drosophila) (DTL) (AA 229-256), (Middle Region) antibody

Details for Product No. ABIN951451
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Antigen
Synonyms CDT2, DCAF2, L2DTL, RAMP, RGD1310439, 2810047L02Rik, 5730564G15Rik, Ramp
Epitope
AA 229-256, Middle Region
(9), (6), (3), (3), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(32), (17), (15), (13), (11), (1), (1), (1)
Host
Rabbit
(31), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(22), (9), (9), (8), (5), (3), (3), (2), (1)
Pubmed 3 references available
Quantity 0.4 mL
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Catalog No. ABIN951451
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Immunogen KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of Human DTL.
Specificity This antibody recognizes Human and Mouse CDT2 / DTL (Center).
Purification Affinity Chromatography on Protein A
Alternative Name CDT2 / DTL
Background Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
Alternate names: CDW1, DCAF2, Ddb1- and Cul4-associated factor 2, Denticleless protein homolog, L2DTL, Lethal(2) denticleless protein homolog, RA regulated Nuclear Matrix Associated Protein, RAMP, Retinoic acid-regulated nuclear matrix-associated protein
Molecular Weight 79468 Da
Gene ID 51514
NCBI Accession NP_057532
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Abbas, Sivaprasad, Terai et al.: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, 2008 (PubMed).

Abbas, Shibata, Park et al.: "CRL4(Cdt2) regulates cell proliferation and histone gene expression by targeting PR-Set7/Set8 for degradation." in: Molecular cell, Vol. 40, Issue 1, pp. 9-21, 2010 (PubMed).

Centore, Havens, Manning et al.: "CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase." in: Molecular cell, Vol. 40, Issue 1, pp. 22-33, 2010 (PubMed).

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