GM-CSF antibody
-
- Target See all GM-CSF (CSF2) Antibodies
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This GM-CSF antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA)
- Specificity
- This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
- Characteristics
- Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
- Purification
- purified
- Sterility
- Sterile filtered
- Immunogen
-
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.
Immunogen Type: RecombinantProtein - Isotype
- IgG
- Top Product
- Discover our top product CSF2 Primary Antibody
-
-
- Application Notes
- This purified antibody has been tested for use in neutralizations, ELISA and western blotting. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 14.5 kDa in size corresponding to human GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
- Comment
-
Gene Name: CSF2
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Expiry Date
- 12 months
-
- Target
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- Alternative Name
- GM-CSF (CSF2 Products)
- Synonyms
- GMCSF antibody, GM-CSF antibody, CSF2 antibody, Csfgm antibody, Gm-CSf antibody, MGI-IGM antibody, Gm-csf antibody, Gmcsf antibody, CSF antibody, colony stimulating factor 2 antibody, colony stimulating factor 2 (granulocyte-macrophage) antibody, CSF2 antibody, Csf2 antibody
- Background
-
Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor; CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
Synonyms: GM-CSF, Colony-stimulating factor, CSF, sargramostim and molgramostin - Gene ID
- 1437
- UniProt
- P04141
- Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
-