IL1RN antibody

Catalog No. ABIN964745

Supplier Product No.: 209-401-303

Product Details anti-IL1RN Antibody

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 209-401-303

Supplier: Rockland

Purpose: IL-1 Receptor Antagonist Antibody

Cross-Reactivity (Details): In general, this antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra.

Characteristics: Synonyms: IL1RA antibody, IL1RN antibody, IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, rabbit anti-IL-1 Receptor Antagonist Antibody, rabbit anti-1 receptor antagonist protein antibody, rabbit anti-IRAP antibody, IL1F3, IL1RA, ICIL-1RA, IL1 inhibitor, INN: Anakinra, IL-1ra, IL-1RN, Interleukin-1 receptor antagonist protein

Purification: This is an IgG preparation of whole rabbit serum purified by DEAE chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested in western blot and suitable for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry and flow cytometry.  Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:400 to 1:800 is suggested for this application.   A working dilution range of 1:500 to 1:1,000 is suggested for ELISA.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.   A dilution of 1:400 or greater is suggested for immunohistochemistry.  Either paraffin fixation or cryofixation can be used for immunohistochemistry.  A dilution of 1:8,000 is suggested for radioimmunoassay against antigen.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL1RN (IL1RN Products)

Background: Background: Interleukin 1 receptor antagonist is secreted by various types of cells including immune, epithelial and adipocytes. IL 1Ra is used to treat rheumatoid arthritis. Anti-IL-1 RA antibody is ideal for investigators involved in Cancer and Immunology research.

Gene ID: 3557

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints