IL1RN antibody

Catalog No. ABIN2856394

This Rabbit Polyclonal antibody specifically detects IL1RN in WB, ICC, IF and IHC (p). It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for IL1RN antibody (ABIN2856394)

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL1RN Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to IL1 Receptor antagonist (interleukin 1 receptor antagonist)
IL1 Receptor antagonist antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human IL1 Receptor antagonist. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A whole , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.86 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-IL1RN antibody (ABIN2856394)

Pfeifer, Thurner, Kessel, Fadle, Kheiroddin, Regitz, Hoffmann, Kos, Preuss, Fischer, Roemer, Lohse, Heyne, Detemple, Fedlmeier, Juenger, Sauer, Meyer, Rohrer, Wittkowski, Becker, Masjosthusmann, Bals et al.: "Autoantibodies against interleukin-1 receptor antagonist in multisystem inflammatory syndrome in children: a multicentre, retrospective, cohort study. ..." in: The Lancet. Rheumatology, Vol. 4, Issue 5, pp. e329-e337, (2022) (PubMed).

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: interleukin 1 receptor antagonist

Background: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Cellular Localization: Isoform 1: Secreted , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 4: Cytoplasm

Molecular Weight: 20 kDa

Gene ID: 3557

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints