B-Cell CLL/lymphoma 10 (BCL10) antibody

Antigen: B-Cell CLL/lymphoma 10 (BCL10)

Synonyms: CLAP, mE10, CIPER, c-E10, CARMEN, ME10, cE10, BCL-10, C81403, AI132454, BCL10, MGC108338

Clonality: Monoclonal

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN965650

Additional Information

Alternative name: BCL-10

Gene ID: 8915

Isotype: IgG1

Description: Bcl-10 is a 31kDa protein containing a caspase recruitment domain (CARD). It plays an important role in apoptosis and activating NF-kappaB. The research suggested that it interacted with other CARD domain containing proteins including CARD9, 10, 11 and 14, which were thought to function as upstream regulators in NF-kappaB signaling. Bcl10 is found to form a complex with MALT1 which encoded by another gene known to be translocated in MALT lymphoma. MALT1 and Bcl-10 are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.

Specificity: Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).

Application Details

Application Notes: IHC(P): Dilution 1: 100- 1: 500
IHC(F): Dilution 1: 200- 1: 1000
Western blot: Dilution 1: 2000- 1: 5000
ELISA: Propose dilution 1: 10000.
Determining optimal working dilutions by titration test.

Purification: Antibodies are purified by protein A affinity chromatography

Storage: Store at -20°C.

Restrictions: For Research Use only

Publications

Product: Cahill: "Vasoconstrictor responsiveness of portal hypertensive vessels." in: Clinical science (London, England : 1979), Vol. 96, Issue 1, pp. 3-4, 1999 (PubMed).

Willis, Jadayel, Du et al.: "Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types." in: Cell, Vol. 96, Issue 1, pp. 35-45, 1999 (PubMed).

Wang, Guo, Huang et al.: "Card10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21405-9, 2001 (PubMed).

Lucas, Yonezumi, Inohara et al.: "Bcl10 and MALT1, independent targets of chromosomal translocation in malt lymphoma, cooperate in a novel NF-kappa B signaling pathway." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 19012-9, 2001 (PubMed).