Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody

Antigen: Fas (TNF Receptor Superfamily, Member 6) (FAS)

Synonyms: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, LOC574159, fasl, zgc:162027, FAS

Clonality: Monoclonal (B500 (B-R18))

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Catalog no.: ABIN965824

Additional Information

Alternative name: CD95

Gene ID: 355

Immunogen: Purified recombinant Fas antigen.

Isotype: IgG1

Clone: B500 (B-R18)

Specificity: This antibody reacts with CD95, a 40-45kD cell surface glycoprotein containing 8kD of N-glycosidic-linked polysaccharide and that is expressed by the CD45RAlow and CD45ROhigh subset of memory T cells.

Comments:

Recommended Positive Control: Tonsil, Spleen, Thymus.

Synonyms: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, LOC574159, fasl, zgc:162027, FAS

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using with formalin-fixed paraffin embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Concentration: 0.3 mg/ml.

Purification: Concentrated.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: 0.05% Sodium Azide

Storage: Store at 2-8° C for short term, freeze under -20°C for long term storage.

Research Area: Immunology, Innate Immunity, Cytokines, CD Antigens, Apoptosis/Necrosis, Death Receptors, Cancer, Growth Factors, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Product: Oehm, Behrmann, Falk et al.: "Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen." in: The Journal of biological chemistry, Vol. 267, Issue 15, pp. 10709-15, 1992 (PubMed).

Itoh, Yonehara, Ishii et al.: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, 1991 (PubMed).

Trauth, Klas, Peters et al.: "Monoclonal antibody-mediated tumor regression by induction of apoptosis." in: Science (New York, N.Y.), Vol. 245, Issue 4915, pp. 301-5, 1989 (PubMed).

Möller, Henne, Leithäuser et al.: "Coregulation of the APO-1 antigen with intercellular adhesion molecule-1 (CD54) in tonsillar B cells and coordinate expression in follicular center B cells and in follicle center and mediastinal B-cell lymphomas." in: Blood, Vol. 81, Issue 8, pp. 2067-75, 1993 (PubMed).

Debatin, Goldman, Waldmann et al.: "APO-1-induced apoptosis of leukemia cells from patients with adult T-cell leukemia." in: Blood, Vol. 81, Issue 11, pp. 2972-7, 1993 (PubMed).