CDW78, , BA ANTIGEN (Leu21) antibody
Reactivity: Human
IHC (fp), IHC (fro), FACS, IF
Host: Mouse
Monoclonal
B351(DF1588)
unconjugated
-
- Target
- CDW78, , BA ANTIGEN
- Binding Specificity
- Leu21
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- Cdw78, also known as Ba antigen, Leu21 or LO-panB-a, is present on some immature and some mature Bcells. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells but not on T-cells, NK cells, monocytes, granulocytes, or myeloid tissues.
- Purification
- Purified.
- Clone
- B351(DF1588)
- Isotype
- IgG1 kappa
-
- Application Notes
-
Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 mg/mL in IHC. - Comment
-
Recommended Positive Control: N/A
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.4 mg/ml.
- Buffer
- 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- CDW78, , BA ANTIGEN
-
You are here:
