Cytokeratin 10+13 (56.5 & 53kDa) antibody

Details for Product No. ABIN965954
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
Pubmed 4 references available
Catalog no. ABIN965954
Quantity 0.5 mL
Price
206.25 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 5 to 7 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Cytoskeleton preparation from HeLa Cells
Clone B28-1 (DE-K13)
Isotype IgG2a
Purification Concentrated.
Background This antibody reacts with Cytokeratin 10 and 13 (56.5 and 53 kD) polypeptide. On formalin-fixed,Paraffinembedded tissue sections, this antibody recognizes only one of the two cytokeratins: Cytokeratin 13, that is found in non-squamous stratified epithelia.
Application Notes Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with Auto ProbeIII. The optimal conditions should be determined by the individual laboratory. Enzymatic predigestion is not recommended.
Comment

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Squamous Granulocytes Carcinomas, Esophagus, or Tongue

Restrictions For Research Use only
Format Liquid
Concentration 0.4 mg/ml
Buffer 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5
Preservative Sodium azide
Storage 4 °C
Product cited in: Ivanyi, Groeneveld, Van Doornewaard et al.: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

van Muijen, Ruiter, Franke et al.: "Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13." in: Experimental cell research, Vol. 162, Issue 1, pp. 97-113, 1986 (PubMed).

Ivanyi, Ansink, Groeneveld et al.: "New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva." in: The Journal of pathology, Vol. 159, Issue 1, pp. 7-12, 1989 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

back to top