Cytokeratin 10+13 (56.5 & 53kDa) antibody

Antigen: Cytokeratin 10+13 (56.5 & 53kDa)

Clonality: Monoclonal (B28-1 (DE-K13))

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN965954

Additional Information

Immunogen: Cytoskeleton preparation from HeLa Cells

Isotype: IgG2a

Clone: B28-1 (DE-K13)

Description: This antibody reacts with Cytokeratin 10 and 13 (56.5 and 53 kD) polypeptide. On formalin-fixed,Paraffinembedded tissue sections, this antibody recognizes only one of the two cytokeratins: Cytokeratin 13, that is found in non-squamous stratified epithelia.

Comments:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Squamous Granulocytes Carcinomas, Esophagus, or Tongue

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with Auto ProbeIII. The optimal conditions should be determined by the individual laboratory. Enzymatic predigestion is not recommended.

Concentration: 0.4 mg/ml

Purification: Concentrated.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: 0.05% Sodium Azide

Storage: Store at 2-8° C for short term, freeze under -20°C for long term storage.

Restrictions: For Research Use only

Publications

Product: Ivanyi, Groeneveld, Van Doornewaard et al.: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

van Muijen, Ruiter, Franke et al.: "Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13." in: Experimental cell research, Vol. 162, Issue 1, pp. 97-113, 1986 (PubMed).

Ivanyi, Ansink, Groeneveld et al.: "New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva." in: The Journal of pathology, Vol. 159, Issue 1, pp. 7-12, 1989 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).