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Cytokeratin 10+13 (56.5 & 53kDa) antibody

Details for Product No. ABIN965954, Supplier: Log in to see
Antigen
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Immunogen Cytoskeleton preparation from HeLa Cells
Clone B28-1 (DE-K13)
Isotype IgG2a
Purification Concentrated.
Background This antibody reacts with Cytokeratin 10 and 13 (56.5 and 53 kD) polypeptide. On formalin-fixed,Paraffinembedded tissue sections, this antibody recognizes only one of the two cytokeratins: Cytokeratin 13, that is found in non-squamous stratified epithelia.
Application Notes Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with Auto ProbeIII. The optimal conditions should be determined by the individual laboratory. Enzymatic predigestion is not recommended.
Comment

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Squamous Granulocytes Carcinomas, Esophagus, or Tongue

Restrictions For Research Use only
Format Liquid
Concentration 0.4 mg/ml
Buffer 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Ivanyi, Ansink, Groeneveld et al.: "New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva." in: The Journal of pathology, Vol. 159, Issue 1, pp. 7-12, 1989 (PubMed).

van Muijen, Ruiter, Franke et al.: "Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13." in: Experimental cell research, Vol. 162, Issue 1, pp. 97-113, 1986 (PubMed).

Ivanyi, Groeneveld, Van Doornewaard et al.: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).