Keratin 10/13 antibody

Catalog No. ABIN3026718

The Mouse Monoclonal anti-Keratin 10/13 antibody is suitable to detect Keratin 10/13 in samples from Human. It has been validated for WB, FACS, IF and IHC (p).

Quick Overview for Keratin 10/13 antibody (ABIN3026718)

Target: Keratin 10/13

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: DE-K13

Product Details

Purpose: Cytokeratin 10/13 Antibody (azide and preservative free)

Characteristics: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Purification: Protein G affinity chromatography

Immunogen: A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10/13 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the Cytokeratin 10/13 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Cytokeratin 10/13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10/13

Background: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on tissue sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

UniProt: P13645