Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2) (C-Term) antibody

Antigen: Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2)

Synonyms: EM9, TTD, XPD, COFS2, MGC102762, MGC126218, MGC126219, Ercc-2, AA407812, AU020867, AW240756, ERCC2, MGC89573, MGC166093

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966088

Additional Information

Alternative name: ERCC2

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human ERCC2(TFIIH basal transcription factor complex helicase subunit)

Description: ERCC2(TFIIH basal transcription factor complex helicase subunit) is an ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. ERCC2 is involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. ERCC2 might also have a role in aging process and could play a causative role in the generation of skin cancers. One of the six subunits forming the core-TFIIH basal transcription factor. The interaction with p44 results in the stimulation of the 5'-->3' helicase activity. Defects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D), xeroderma pigmentosum group D combined with Cockayne syndrome (XP-D/CS). Defects in ERCC2 are a cause of trichothiodystrophy (TTD) and COFS syndrome. ERCC2 belongs to the helicase family and RAD3/XPD subfamily.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Restrictions: For Research Use only

Publications

Product: Flejter, McDaniel, Johns et al.: "Correction of xeroderma pigmentosum complementation group D mutant cell phenotypes by chromosome and gene transfer: involvement of the human ERCC2 DNA repair gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 1, pp. 261-5, 1992 (PubMed).

Weber, Salazar, Stewart et al.: "ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3." in: The EMBO journal, Vol. 9, Issue 5, pp. 1437-47, 1990 (PubMed).

Takayama, Salazar, Lehmann et al.: "Defects in the DNA repair and transcription gene ERCC2 in the cancer-prone disorder xeroderma pigmentosum group D." in: Cancer research, Vol. 55, Issue 23, pp. 5656-63, 1995 (PubMed).

Sung, Bailly, Weber et al.: "Human xeroderma pigmentosum group D gene encodes a DNA helicase." in: Nature, Vol. 365, Issue 6449, pp. 852-5, 1993 (PubMed).

Taylor, Broughton, Botta et al.: "Xeroderma pigmentosum and trichothiodystrophy are associated with different mutations in the XPD (ERCC2) repair/transcription gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 16, pp. 8658-63, 1997 (PubMed).

Coin, Marinoni, Rodolfo et al.: "Mutations in the XPD helicase gene result in XP and TTD phenotypes, preventing interaction between XPD and the p44 subunit of TFIIH." in: Nature genetics, Vol. 20, Issue 2, pp. 184-8, 1998 (PubMed).

Tirode, Busso, Coin et al.: "Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7." in: Molecular cell, Vol. 3, Issue 1, pp. 87-95, 1999 (PubMed).

Spitz, Wu, Wang et al.: "Modulation of nucleotide excision repair capacity by XPD polymorphisms in lung cancer patients." in: Cancer research, Vol. 61, Issue 4, pp. 1354-7, 2001 (PubMed).

Caggana, Kilgallen, Conroy et al.: "Associations between ERCC2 polymorphisms and gliomas." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 10, Issue 4, pp. 355-60, 2001 (PubMed).