anti-Exonuclease 1 antibody (EXO1) (N-Term)

Details for Product anti-EXO1 Antibody No. ABIN966108
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Antigen
Synonyms ATWEX, ATWRNEXO, EXONUCLEASE, F18A5.260, F18A5_260, WEX, Werner syndrome-like exonuclease, EXO1, HEX1, hExoI, 5730442G03Rik, Msa, fc16e04, wu:fc16e04, zgc:55521, F15D2.37, F15D2_37, exoi
Epitope
N-Term
(10), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(74), (30), (12), (1)
Host
Rabbit
(47), (20), (18)
Clonality
Polyclonal
Conjugate
This Exonuclease 1 antibody is un-conjugated
(4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(66), (35), (18), (10), (6), (5), (3), (2), (2), (1), (1)
Pubmed 15 references available
Quantity 0.1 mg
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Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of human exonuclease 1
Purification Purified by antigen-specific affinity chromatography.
Alternative Name EXO1 (EXO1 Antibody Abstract)
Background EXO1 (exonuclease 1) is a 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatchcontaining DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5' overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5' end of a downstream Okazaki fragment. EXO1 is required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. It is essential for male and female meiosis. EXO1 interacts with the MLH1-PMS2 heterodimer via MLH1. It interacts with MSH3 and with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. EXO1 interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. EXO1 interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5' overhanging flap structures. It also interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5' overhanging flap structures.
Research Area Metabolism
Application Notes ELISA, Western blotting: 1µg/ml for 2hrs.
Restrictions For Research Use only
Format Liquid
Buffer This antibody is stored in PBS, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Genschel, Modrich: "Functions of MutLalpha, replication protein A (RPA), and HMGB1 in 5'-directed mismatch repair." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21536-44, 2009 (PubMed).

Alam, Gorman, Jaeger et al.: "Germline deletions of EXO1 do not cause colorectal tumors and lesions which are null for EXO1 do not have microsatellite instability." in: Cancer genetics and cytogenetics, Vol. 147, Issue 2, pp. 121-7, 2003 (PubMed).

Sharma, Sommers, Driscoll et al.: "The exonucleolytic and endonucleolytic cleavage activities of human exonuclease 1 are stimulated by an interaction with the carboxyl-terminal region of the Werner syndrome protein." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23487-96, 2003 (PubMed).

Background publications Zhang, Yuan, Presnell et al.: "Reconstitution of 5'-directed human mismatch repair in a purified system." in: Cell, Vol. 122, Issue 5, pp. 693-705, 2005 (PubMed).

Dzantiev, Constantin, Genschel et al.: "A defined human system that supports bidirectional mismatch-provoked excision." in: Molecular cell, Vol. 15, Issue 1, pp. 31-41, 2004 (PubMed).

Nielsen, Jäger, Lützen et al.: "Characterization of human exonuclease 1 in complex with mismatch repair proteins, subcellular localization and association with PCNA." in: Oncogene, Vol. 23, Issue 7, pp. 1457-68, 2004 (PubMed).

Sun, Zheng, Shen: "Functional alterations of human exonuclease 1 mutants identified in atypical hereditary nonpolyposis colorectal cancer syndrome." in: Cancer research, Vol. 62, Issue 21, pp. 6026-30, 2002 (PubMed).

Genschel, Bazemore, Modrich: "Human exonuclease I is required for 5' and 3' mismatch repair." in: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 13302-11, 2002 (PubMed).

Jäger, Rasmussen, Bisgaard et al.: "HNPCC mutations in the human DNA mismatch repair gene hMLH1 influence assembly of hMutLalpha and hMLH1-hEXO1 complexes." in: Oncogene, Vol. 20, Issue 27, pp. 3590-5, 2001 (PubMed).

Schmutte, Sadoff, Shim et al.: "The interaction of DNA mismatch repair proteins with human exonuclease I." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 33011-8, 2001 (PubMed).

Rasmussen, Rasmussen, Lee et al.: "Identification of factors interacting with hMSH2 in the fetal liver utilizing the yeast two-hybrid system. In vivo interaction through the C-terminal domains of hEXO1 and hMSH2 and comparative ..." in: Mutation research, Vol. 460, Issue 1, pp. 41-52, 2000 (PubMed).

Lee, Wilson: "The RAD2 domain of human exonuclease 1 exhibits 5' to 3' exonuclease and flap structure-specific endonuclease activities." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37763-9, 2000 (PubMed).

Qiu, Qian, Chen et al.: "Human exonuclease 1 functionally complements its yeast homologues in DNA recombination, RNA primer removal, and mutation avoidance." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17893-900, 1999 (PubMed).

Tishkoff, Amin, Viars et al.: "Identification of a human gene encoding a homologue of Saccharomyces cerevisiae EXO1, an exonuclease implicated in mismatch repair and recombination." in: Cancer research, Vol. 58, Issue 22, pp. 5027-31, 1998 (PubMed).

Schmutte, Marinescu, Sadoff et al.: "Human exonuclease I interacts with the mismatch repair protein hMSH2." in: Cancer research, Vol. 58, Issue 20, pp. 4537-42, 1998 (PubMed).

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Catalog No. ABIN966108
330.00 $
Plus shipping costs $45.00
Quantity
Price
0.1 mg
330.00 $

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