Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (AA 400-500) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4)

Synonyms: HGK, NIK, FLH21957, FLJ10410, FLJ20373, FLJ90111, KIAA0687

Binding Site: AA 400-500

Clonality: Monoclonal (3C7B5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN966520

Additional Information

Alternative name: MAP4K4

Gene ID: 9448

Immunogen: Purified recombinant fragment of MAP4K4 (aa400-500) expressed in E. Coli.

Isotype: IgG1

Clone: 3C7B5

Description: MAP4K4: mitogen-activated protein kinase kinase kinase kinase 4. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Synonyms: HGK, NIK

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascitic fluid

Preservative: 0.03% sodium azide.

Storage: Store at 4°C, for long term storage, store at -20°C.

Research Area: Signaling

Restrictions: For Research Use only

Publications

Product: Becker, Huynh-Do, Holland et al.: "Nck-interacting Ste20 kinase couples Eph receptors to c-Jun N-terminal kinase and integrin activation." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1537-45, 2000 (PubMed).

Lantinga van Leeuwen, Teske, van Garderen et al.: "Growth hormone gene expression in normal lymph nodes and lymphomas of the dog." in: Anticancer research, Vol. 20, Issue 4, pp. 2371-6, 2000 (PubMed).

Poinat, De Arcangelis, Sookhareea et al.: "A conserved interaction between beta1 integrin/PAT-3 and Nck-interacting kinase/MIG-15 that mediates commissural axon navigation in C. elegans." in: Current biology : CB, Vol. 12, Issue 8, pp. 622-31, 2002 (PubMed).

Bouzakri, Zierath: "MAP4K4 gene silencing in human skeletal muscle prevents tumor necrosis factor-alpha-induced insulin resistance." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 7783-9, 2007 (PubMed).