IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody
|Synonyms||EMT, LYK, PSCTK2, MGC126257, MGC126258, Emt, Tsk, Tcsk|
Human, Mouse (Murine)
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Kinase Activity Assay (KAA)
|3 references available|
|Quantity||50 µg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Fusion protein with of human/mouse Itk|
|Description||Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||72 kDa|
Related Products: ABIN968537
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Research Area||Signaling, Tyrosine Kinases|
|Restrictions||For Research Use only|
Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, 1993 (PubMed).
Heyeck, Wilcox, Bunnell et al.: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, 1997 (PubMed).
Liu, Bunnell, Gurniak et al.: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, 1998 (PubMed).
|Hosts||Rabbit (30), Mouse (9), Goat (5)|
|Reactivities||Human (40), Mouse (Murine) (25), Rat (Rattus) (21), Chicken (19), Cow (Bovine) (19), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (24), ELISA (21), Immunofluorescence (IF) (17), Immunohistochemistry (IHC) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (3), pTyr512,C-Term (3), AA 1-26 (1), AA 1-270 (1), AA 2-110 (1), AA 596-607 (1), Center (1), Internal Region (1), N-Term (1)|