IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody

Details for Product No. ABIN967287
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms EMT, LPFS1, LYK, PSCTK2, Emt, Tsk, Tcsk
Epitope
AA 1-26
(9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(68), (24), (17), (13), (12), (12), (12)
Host
Mouse
(35), (25), (13)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Kinase Activity Assay (KAA)
(56), (31), (10), (10), (8), (6), (6), (4), (3), (2), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN967287
Quantity 50 µg
Price
Contact our Customer Service for availability and price in your country.
Options
Shipping to United States (Change)
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Fusion protein with of human/mouse Itk
Clone 2F12
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name ITK
Background Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
Molecular Weight 72 kDa
Research Area Signaling, Tyrosine Kinases
Comment

Related Products: ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clone 2F12, ABIN967287) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 myg/ml (lane 3). Itk is identified as a band at ~72 kDa.
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody (2) Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 myg/1 x 10^6 cells) with anti-Itk (clone 2F12, ABIN967287) and then the western blot was probed with the same antibody at a concentration of 0.5 myg/ml. Itk is identified as a band of ~72 kDa.
Product cited in: Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, 1993 (PubMed).

Heyeck, Wilcox, Bunnell et al.: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, 1997 (PubMed).

Liu, Bunnell, Gurniak et al.: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, 1998 (PubMed).

Hosts (35), (25), (13)
Reactivities (68), (24), (17), (13), (12), (12), (12)
Applications (56), (31), (10), (10), (8), (6), (6), (4), (3), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top