IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody

Antigen: IL2-Inducible T-Cell Kinase (ITK)

Synonyms: EMT, LYK, PSCTK2, MGC126257, MGC126258, Emt, Tsk, Tcsk

Binding Site: AA 1-26

Clonality: Monoclonal (2F12)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Kinase Activity Assay (KAA)

Catalog no.: ABIN967287

Additional Information

Alternative name: ITK

Immunogen: Fusion protein with of human/mouse Itk

Format: Liquid

Isotype: IgG1

Clone: 2F12

Description: Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 72 kDa

Comments:

Related Products: ABIN968537

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clone 2F12, ABIN967287) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 myg/ml (lane 3). Itk is identified as a band at ~72 kDa.

Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 myg/1 x 10^6 cells) with anti-Itk (clone 2F12, ABIN967287) and then the western blot was probed with the same antibody at a concentration of 0.5 myg/ml. Itk is identified as a band of ~72 kDa.

Publications

Product: Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, 1993 (PubMed).

Heyeck, Wilcox, Bunnell et al.: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, 1997 (PubMed).

Liu, Bunnell, Gurniak et al.: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, 1998 (PubMed).