IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody

Details for Product No. ABIN967287
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Antigen
Synonyms Emt, Tcsk, Tsk, EMT, LPFS1, LYK, PSCTK2
Epitope
AA 1-26
(28), (19), (16), (14), (8), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(113), (31), (27), (3), (1), (1), (1), (1)
Host
Mouse
(68), (33), (23)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunoprecipitation (IP), Kinase Activity Assay (KAA), Western Blotting (WB)
(100), (67), (10), (8), (7), (6), (6), (5), (4), (4), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 50 μg
Options
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Immunogen Fusion protein with of human/mouse Itk
Clone 2F12
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ITK (ITK Antibody Abstract)
Background Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
Molecular Weight 72 kDa
Research Area Signaling, Tyrosine Kinases
Comment

Related Products: ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clon...
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody (2) Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were firs...
Product cited in: Liu, Bunnell, Gurniak et al.: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, 1998 (PubMed).

Heyeck, Wilcox, Bunnell et al.: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, 1997 (PubMed).

Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, 1993 (PubMed).

Validation Images
Catalog No. ABIN967287
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