ITK antibody (IL2-Inducible T-Cell Kinase) (C-Term)

Details for Product anti-ITK Antibody No. ABIN184605
Target
Binding Specificity
C-Term
16
15
9
9
7
7
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
70
20
13
Host
Goat
65
8
7
Clonality
Polyclonal
Conjugate
This ITK antibody is un-conjugated
9
6
5
5
5
5
2
2
2
2
2
2
Application
ELISA, Immunofluorescence (IF), Western Blotting (WB)
67
50
10
6
5
3
2
2
2
2
1
1
Options
Purpose ITK
Immunogen Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.
Blocking Peptide Blocking peptide for this product available: ABIN369790
Sequence RLLRQLAEIA ESGL
Isotype IgG
Predicted Reactivity Human, Mouse
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name ITK (ITK Antibody Abstract)
Background ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258
Gene ID 3702
NCBI Accession NP_005537
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes

DS WB Results: Approx 75 kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8 kDa according to NP_005537.3). Recommended concentration: 1-3g/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentration:of 10ug/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-IL2-Inducible T-Cell Kinase (ITK) (C-Term) antibody (ABIN184605) ABIN184605 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permea...
Image no. 2 for anti-IL2-Inducible T-Cell Kinase (ITK) (C-Term) antibody (ABIN184605) ABIN184605 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). ...
Image no. 3 for anti-IL2-Inducible T-Cell Kinase (ITK) (C-Term) antibody (ABIN184605) HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human (ITK) with DYKDDDDK ...
Image no. 4 for anti-IL2-Inducible T-Cell Kinase (ITK) (C-Term) antibody (ABIN184605) ABIN184605 (1ug/ml) staining of Jurkat nuclear cell lysate (A) and negative control H...
Image no. 5 for anti-IL2-Inducible T-Cell Kinase (ITK) (C-Term) antibody (ABIN184605) ABIN184605 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabi...