Sialophorin (SPN) antibody
|Synonyms||LSN, CD43, GPL115|
Alternatives Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC)
|9 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Alternative name||CD43 (Leukosialin)|
|Description||Anti-leukosialin monoclonal antibody (clone L60) recognizes a sialic acid-dependent determinant on leukosialin, a strongly anionic, highly o-linked glycosylated cell surface protein with a 45 nm extracellular domain. Leukosialin, also know as sialophorin or CD43, is a major component of T lymphocyte, thymocyte, and neutrophil (PMN) membranes, and is also expressed on many different cells including monocytes, platelets, bone marrow stem cells, and plasma cells.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Storage||Store undiluted at 4° C.|
|Research Area||CD Antigens|
|Restrictions||For Research Use only|
|Profile of CD43 (L60) reactivity on peripheral blood lymphocytes analyzed by flow cytometry. Second step staining.|
Kuijpers, Hoogerwerf, Kuijpers et al.: "Cross-linking of sialophorin (CD43) induces neutrophil aggregation in a CD18-dependent and a CD18-independent way." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 998-1003, 1992 (PubMed).
Rieu, Porteu, Bessou et al.: "Human neutrophils release their major membrane sialoprotein, leukosialin (CD43), during cell activation." in: European journal of immunology, Vol. 22, Issue 11, pp. 3021-6, 1992 (PubMed).
Cyster, Williams: "The importance of cross-linking in the homotypic aggregation of lymphocytes induced by anti-leukosialin (CD43) antibodies." in: European journal of immunology, Vol. 22, Issue 10, pp. 2565-72, 1992 (PubMed).
Ardman, Sikorski, Staunton: "CD43 interferes with T-lymphocyte adhesion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 11, pp. 5001-5, 1992 (PubMed).
Segal, Stoler, Tubbs: "The "CD43 only" phenotype. An aberrant, nonspecific immunophenotype requiring comprehensive analysis for lineage resolution." in: American journal of clinical pathology, Vol. 97, Issue 6, pp. 861-5, 1992 (PubMed).
Rosenstein, Park, Hahn et al.: "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1." in: Nature, Vol. 354, Issue 6350, pp. 233-5, 1992 (PubMed).
Campanero, Pulido, Alonso et al.: "Down-regulation by tumor necrosis factor-alpha of neutrophil cell surface expression of the sialophorin CD43 and the hyaluronate receptor CD44 through a proteolytic mechanism." in: European journal of immunology, Vol. 21, Issue 12, pp. 3045-8, 1992 (PubMed).
Park, Rosenstein, Remold-ODonnell et al.: "Enhancement of T-cell activation by the CD43 molecule whose expression is defective in Wiskott-Aldrich syndrome." in: Nature, Vol. 350, Issue 6320, pp. 706-9, 1991 (PubMed).
Bazil, Strominger: "CD43, the major sialoglycoprotein of human leukocytes, is proteolytically cleaved from the surface of stimulated lymphocytes and granulocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 3792-6, 1993 (PubMed).