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CD43 antibody (Sialophorin)

Details for Product anti-SPN Antibody No. ABIN967347, Supplier: Login to see
Antigen
  • CD43
  • GALGP
  • GPL115
  • LSN
  • Cd43
  • Lsn
  • Lsn1
  • gpL115
  • A630014B01Rik
  • Galgp
  • Ly-48
  • Ly48
Reactivity
Human
360
64
59
3
3
1
1
Host
Mouse
317
108
56
Clonality (Clone)
Monoclonal ()
Conjugate
This CD43 antibody is un-conjugated
61
59
40
21
9
9
6
3
3
3
2
2
2
2
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
300
197
128
114
77
55
53
38
20
8
8
3
3
2
2
1
1
1
Supplier
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Brand BD Pharmingen™
Clone L60
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD43 (Leukosialin) (SPN Antibody Abstract)
Background Anti-leukosialin monoclonal antibody (clone L60) recognizes a sialic acid-dependent determinant on leukosialin, a strongly anionic, highly o-linked glycosylated cell surface protein with a 45 nm extracellular domain. Leukosialin, also know as sialophorin or CD43, is a major component of T lymphocyte, thymocyte, and neutrophil (PMN) membranes, and is also expressed on many different cells including monocytes, platelets, bone marrow stem cells, and plasma cells.
Research Area CD Antigens
Pathways
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing no preservative.
Preservative Without preservative
Storage 4 °C
Storage Comment This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4° C.
Supplier Images
 image for anti-CD43 antibody (Sialophorin) (ABIN967347) Profile of CD43 (L60) reactivity on peripheral blood lymphocytes analyzed by flow cyt...
Product cited in: Bazil, Strominger: "CD43, the major sialoglycoprotein of human leukocytes, is proteolytically cleaved from the surface of stimulated lymphocytes and granulocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 3792-6, 1993 (PubMed).

Park, Rosenstein, Remold-ODonnell et al.: "Enhancement of T-cell activation by the CD43 molecule whose expression is defective in Wiskott-Aldrich syndrome." in: Nature, Vol. 350, Issue 6320, pp. 706-9, 1991 (PubMed).

Campanero, Pulido, Alonso et al.: "Down-regulation by tumor necrosis factor-alpha of neutrophil cell surface expression of the sialophorin CD43 and the hyaluronate receptor CD44 through a proteolytic mechanism." in: European journal of immunology, Vol. 21, Issue 12, pp. 3045-8, 1992 (PubMed).

Rosenstein, Park, Hahn et al.: "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1." in: Nature, Vol. 354, Issue 6350, pp. 233-5, 1992 (PubMed).

Segal, Stoler, Tubbs: "The "CD43 only" phenotype. An aberrant, nonspecific immunophenotype requiring comprehensive analysis for lineage resolution." in: American journal of clinical pathology, Vol. 97, Issue 6, pp. 861-5, 1992 (PubMed).

Ardman, Sikorski, Staunton: "CD43 interferes with T-lymphocyte adhesion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 11, pp. 5001-5, 1992 (PubMed).

Cyster, Williams: "The importance of cross-linking in the homotypic aggregation of lymphocytes induced by anti-leukosialin (CD43) antibodies." in: European journal of immunology, Vol. 22, Issue 10, pp. 2565-72, 1992 (PubMed).

Rieu, Porteu, Bessou et al.: "Human neutrophils release their major membrane sialoprotein, leukosialin (CD43), during cell activation." in: European journal of immunology, Vol. 22, Issue 11, pp. 3021-6, 1992 (PubMed).

Kuijpers, Hoogerwerf, Kuijpers et al.: "Cross-linking of sialophorin (CD43) induces neutrophil aggregation in a CD18-dependent and a CD18-independent way." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 998-1003, 1992 (PubMed).