Sialophorin (SPN) antibody

Antigen: Sialophorin (SPN)

Synonyms: LSN, CD43, GPL115

Clonality: Monoclonal (L60)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN967347

Additional Information

Alternative name: CD43 (Leukosialin)

Format: Liquid

Isotype: IgG1, kappa

Clone: L60

Description: Anti-leukosialin monoclonal antibody (clone L60) recognizes a sialic acid-dependent determinant on leukosialin, a strongly anionic, highly o-linked glycosylated cell surface protein with a 45 nm extracellular domain. Leukosialin, also know as sialophorin or CD43, is a major component of T lymphocyte, thymocyte, and neutrophil (PMN) membranes, and is also expressed on many different cells including monocytes, platelets, bone marrow stem cells, and plasma cells.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: No preservative.

Storage: Store undiluted at 4° C.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

Profile of CD43 (L60) reactivity on peripheral blood lymphocytes analyzed by flow cytometry. Second step staining.

Publications

Product: Kuijpers, Hoogerwerf, Kuijpers et al.: "Cross-linking of sialophorin (CD43) induces neutrophil aggregation in a CD18-dependent and a CD18-independent way." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 998-1003, 1992 (PubMed).

Rieu, Porteu, Bessou et al.: "Human neutrophils release their major membrane sialoprotein, leukosialin (CD43), during cell activation." in: European journal of immunology, Vol. 22, Issue 11, pp. 3021-6, 1992 (PubMed).

Cyster, Williams: "The importance of cross-linking in the homotypic aggregation of lymphocytes induced by anti-leukosialin (CD43) antibodies." in: European journal of immunology, Vol. 22, Issue 10, pp. 2565-72, 1992 (PubMed).

Ardman, Sikorski, Staunton: "CD43 interferes with T-lymphocyte adhesion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 11, pp. 5001-5, 1992 (PubMed).

Segal, Stoler, Tubbs: "The "CD43 only" phenotype. An aberrant, nonspecific immunophenotype requiring comprehensive analysis for lineage resolution." in: American journal of clinical pathology, Vol. 97, Issue 6, pp. 861-5, 1992 (PubMed).

Rosenstein, Park, Hahn et al.: "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1." in: Nature, Vol. 354, Issue 6350, pp. 233-5, 1992 (PubMed).

Campanero, Pulido, Alonso et al.: "Down-regulation by tumor necrosis factor-alpha of neutrophil cell surface expression of the sialophorin CD43 and the hyaluronate receptor CD44 through a proteolytic mechanism." in: European journal of immunology, Vol. 21, Issue 12, pp. 3045-8, 1992 (PubMed).

Park, Rosenstein, Remold-ODonnell et al.: "Enhancement of T-cell activation by the CD43 molecule whose expression is defective in Wiskott-Aldrich syndrome." in: Nature, Vol. 350, Issue 6320, pp. 706-9, 1991 (PubMed).

Bazil, Strominger: "CD43, the major sialoglycoprotein of human leukocytes, is proteolytically cleaved from the surface of stimulated lymphocytes and granulocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 3792-6, 1993 (PubMed).