CD43 antibody (Sialophorin)

Details for Product anti-SPN Antibody No. ABIN6940632
Antigen
  • CD43
  • GALGP
  • GPL115
  • LSN
  • A630014B01Rik
  • Cd43
  • Galgp
  • Ly-48
  • Ly48
  • Lsn
  • Lsn1
  • gpL115
  • sialophorin
  • SPN
  • Spn
Reactivity
Human
528
44
27
2
1
Host
Mouse
410
160
14
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
This CD43 antibody is un-conjugated
52
47
41
22
16
15
10
6
6
6
6
6
6
6
6
6
6
6
6
6
3
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
397
255
214
205
118
107
82
54
36
21
19
5
3
2
1
Options
Immunogen Recombinant full-length human CD43 protein
Clone RSPN-839
Isotype IgG1, kappa
Specificity It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb rSPN/839, called b as opposed to a for rSPN/839. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Purification Purified by Protein A/G
Plasmids, Primers & others Plasmids, Primers & others CD43 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name SPN (SPN Antibody Abstract)
Molecular Weight 95, 115, or 135kDa
Gene ID 6693
UniProt P16150
Pathways Regulation of Leukocyte Mediated Immunity
Application Notes

Positive Control: K562 cells. Paracortex in a tonsil or a reactive lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions For Research Use only
Concentration 200 μg/mL
Buffer 10 mM PBS with 0.05 % BSA & 0.05 % azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-80 °C
Storage Comment Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Expiry Date 24 months
Supplier Images
Image no. 1 for anti-Sialophorin (SPN) antibody (ABIN6940632) Western Blot Analysis of K562 cell lysate using CD43 Mouse Recombinant Monoclonal Ant...
Image no. 2 for anti-Sialophorin (SPN) antibody (ABIN6940632) Flow Cytometric Analysis of PFA-fixed K562 cells using CD43 Mouse Recombinant Monoclo...
Image no. 3 for anti-Sialophorin (SPN) antibody (ABIN6940632) Formalin-fixed, paraffin-embedded human Lymph Node stained with CD43 Mouse Recombinan...
Image no. 4 for anti-Sialophorin (SPN) antibody (ABIN6940632) Immunofluorescence Analysis of K562 cells labeling CD43 with CD43 Mouse Recombinant M...
Image no. 5 for anti-Sialophorin (SPN) antibody (ABIN6940632) SDS-PAGE Analysis Purified CD43 Mouse Recombinant Monoclonal Antibody (rSPN/839). Con...
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