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Notch1 antibody (Notch 1)

Details for Product anti-NOTCH1 Antibody No. ABIN967360, Supplier: Login to see New
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Synonyms notch1, NOTCH1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1, TAN1, hN1, 9930111A19Rik, Mis6, N1, Tan1, lin-12, NOTCH, Xotch, notch1-a
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Human, Mouse (Murine)
(206), (150), (60), (6), (2), (2), (2), (1), (1), (1)
(141), (129), (15), (8), (7), (2), (1)
Clonality (Clone)
Monoclonal ()
This Notch1 antibody is un-conjugated
(23), (14), (13), (7), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Intracellular Staining (ICS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(186), (91), (78), (78), (52), (37), (32), (16), (15), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Quantity 0.1 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Mouse Notch1 GST fusion protein
Clone MN1A
Isotype IgG1, kappa
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. An isotype control should be used at the same concentration as the antibody of interest.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Notch1 (NOTCH1 Antibody Abstract)
Background The Notch family of transmembrane receptors controls cell-fate decisions during the development of many organs in a wide variety of animal species. After binding its ligand, a Notch receptor is cleaved in its transmembrane domain, and the resulting intracellular domain dissociates from the membrane and translocates to the nucleus, where it is able to suppress the expression of lineage-specific genes by interacting with transcriptional repressors. The mN1A antibody reacts with the intracellular domain of mouse and human Notch1, but not with mouse Notch2, 3, or 4. In the mouse, Notch1 mRNA is expressed in mouse hematopoietic cells of the fetal liver and adult thymus and bone marrow. Using mAb mN1A, Notch1 is detected in CD4-CD8- (double-negative) and CD4-CD8+ thymocytes. Studies of Notch1-transgenic cells and Notch1-null mice indicate that the receptor is involved in the regulation of lymphopoiesis and myelopoiesis. The mN1A mAb does not cross-react with rat thymocytes. An alternative anti-mouse Notch1 monoclonal antibody, clone 22E5.5, specifically binds to an extracellular domain of mouse Notch1.
Synonyms: Notch1, Notch 1, NOTC1, lin-12, Mis6, Motch A, mT14, N1, NICD, p300, Tan1
Research Area Embryogenesis, Cardiogenesis, Hematopoietic Progenitors, Transcription Factors
Pathways Notch Signaling
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Flow Cytometry (FACS) image for anti-Notch1 antibody (Notch 1) (ABIN967360) Expression of Notch1 in double-negative thymocytes. Fixed and permeabilized C57BL/6 t...
Product cited in: Kawamata, Du, Li et al.: "Notch1 perturbation of hemopoiesis involves non-cell- autonomous modifications." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1738-45, 2002 (PubMed).

Walker, Carlson, Tan-Pertel et al.: "The notch receptor and its ligands are selectively expressed during hematopoietic development in the mouse." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 6, pp. 543-52, 2001 (PubMed).

Anderson, Robey, Huang: "Notch signaling in lymphocyte development." in: Current opinion in genetics & development, Vol. 11, Issue 5, pp. 554-60, 2001 (PubMed).

Kojika, Griffin: "Notch receptors and hematopoiesis." in: Experimental hematology, Vol. 29, Issue 9, pp. 1041-52, 2001 (PubMed).

Huppert, Le, Schroeter et al.: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, 2000 (PubMed).

Artavanis-Tsakonas, Rand, Lake: "Notch signaling: cell fate control and signal integration in development." in: Science (New York, N.Y.), Vol. 284, Issue 5415, pp. 770-6, 1999 (PubMed).

Milner, Bigas: "Notch as a mediator of cell fate determination in hematopoiesis: evidence and speculation." in: Blood, Vol. 93, Issue 8, pp. 2431-48, 1999 (PubMed).

Milner, Bigas, Kopan et al.: "Inhibition of granulocytic differentiation by mNotch1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 13014-9, 1996 (PubMed).

Catalog No. ABIN967360
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