Notch 1 (NOTCH1) antibody

Antigen: Notch 1 (NOTCH1)

Synonyms: hN1, TAN1, Mis6, Tan1, lin-12, 9930111A19Rik, NOTCH, NOTCH1, notch1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1

Clonality: Monoclonal (MN1A)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Intracellular Flow Cytometry (ICFC), ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN967360

Additional Information

Alternative name: Notch1

Immunogen: Mouse Notch1 GST fusion protein

Cross-Reactivity: Human

Format: Liquid

Isotype: IgG1, kappa

Clone: MN1A

Description: The Notch family of transmembrane receptors controls cell-fate decisions during the development of many organs in a wide variety of animal species. After binding its ligand, a Notch receptor is cleaved in its transmembrane domain, and the resulting intracellular domain dissociates from the membrane and translocates to the nucleus, where it is able to suppress the expression of lineage-specific genes by interacting with transcriptional repressors. The mN1A antibody reacts with the intracellular domain of mouse and human Notch1, but not with mouse Notch2, 3, or 4. In the mouse, Notch1 mRNA is expressed in mouse hematopoietic cells of the fetal liver and adult thymus and bone marrow. Using mAb mN1A, Notch1 is detected in CD4-CD8- (double-negative) and CD4-CD8+ thymocytes. Studies of Notch1-transgenic cells and Notch1-null mice indicate that the receptor is involved in the regulation of lymphopoiesis and myelopoiesis. The mN1A mAb does not cross-react with rat thymocytes. An alternative anti-mouse Notch1 monoclonal antibody, clone 22E5.5, specifically binds to an extracellular domain of mouse Notch1.
Synonyms: Notch1, Notch 1, NOTC1, lin-12, Mis6, Motch A, mT14, N1, NICD, p300, Tan1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. An isotype control should be used at the same concentration as the antibody of interest.

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Research Area: Embryogenesis, Cardiogenesis, Hematopoietic Progenitors, Transcription Factors

Restrictions: For Research Use only

Images

Expression of Notch1 in double-negative thymocytes. Fixed and permeabilized C57BL/6 thymocytes were stained with either Purified Mouse anti-Mouse Notch1 (filled histogram) or Purified Mouse IgG1, kappa Isotype Control (open histogram), followed by FITC Rat anti-Mouse IgG1. Cells were then stained with APC Rat anti-Mouse CD4 and PE Rat anti-Mouse CD8a. For analysis of Notch1 expression, an electronic gate was set to include only the CD4-CD8a- subpopulation. Flow cytometry was performed on a BD FACSCalibur™ Flow Cytometry System.

Publications

Product: Milner, Bigas, Kopan et al.: "Inhibition of granulocytic differentiation by mNotch1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 13014-9, 1996 (PubMed).

Milner, Bigas: "Notch as a mediator of cell fate determination in hematopoiesis: evidence and speculation." in: Blood, Vol. 93, Issue 8, pp. 2431-48, 1999 (PubMed).

Artavanis-Tsakonas, Rand, Lake: "Notch signaling: cell fate control and signal integration in development." in: Science (New York, N.Y.), Vol. 284, Issue 5415, pp. 770-6, 1999 (PubMed).

Huppert, Le, Schroeter et al.: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, 2000 (PubMed).

Kojika, Griffin: "Notch receptors and hematopoiesis." in: Experimental hematology, Vol. 29, Issue 9, pp. 1041-52, 2001 (PubMed).

Anderson, Robey, Huang: "Notch signaling in lymphocyte development." in: Current opinion in genetics & development, Vol. 11, Issue 5, pp. 554-60, 2001 (PubMed).

Walker, Carlson, Tan-Pertel et al.: "The notch receptor and its ligands are selectively expressed during hematopoietic development in the mouse." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 6, pp. 543-52, 2001 (PubMed).

Kawamata, Du, Li et al.: "Notch1 perturbation of hemopoiesis involves non-cell- autonomous modifications." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1738-45, 2002 (PubMed).