Sialophorin (SPN) antibody

Antigen: Sialophorin (SPN)

Synonyms: LSN, CD43, GPL115

Clonality: Monoclonal (S7)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Co-Stimulation (CoSt)

Catalog no.: ABIN967373

Additional Information

Alternative name: CD43 (Leukosialin)

Immunogen: Mouse Plasmacytoma MOPC-315

Format: Liquid

Isotype: IgG2a, kappa

Clone: S7

Description: The S7 antibody reacts with the 115 kDa glycoform of CD43 (Ly-48, Leukosialin) which is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Ly-48, Leukosialin

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Research Area: CD Antigens

Restrictions: For Research Use only

Publications

Hardy, Hayakawa: "Generation of Ly-1 B cells from developmentally distinct precursors. Enrichment by stromal-cell culture or cell sorting." in: Annals of the New York Academy of Sciences, Vol. 651, pp. 99-111, 1992 (PubMed).

Hardy, Carmack, Shinton et al.: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, 1991 (PubMed).

Gulley, Ogata, Thorson et al.: "Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3751-7, 1988 (PubMed).

Jones, Federsppiel, Ellies et al.: "Characterization of the activation-associated isoform of CD43 on murine T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3426-39, 1994 (PubMed).

Moore, Huang, Terstappen et al.: "Expression of CD43 on murine and human pluripotent hematopoietic stem cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4978-87, 1994 (PubMed).

Moore, Bennett, Kumar: "Transplantable NK cell progenitors in murine bone marrow." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1653-63, 1995 (PubMed).

Manjunath, Correa, Ardman et al.: "Negative regulation of T-cell adhesion and activation by CD43." in: Nature, Vol. 377, Issue 6549, pp. 535-8, 1995 (PubMed).

Sperling, Green, Mosley et al.: "CD43 is a murine T cell costimulatory receptor that functions independently of CD28." in: The Journal of experimental medicine, Vol. 182, Issue 1, pp. 139-46, 1995 (PubMed).

Wells, Kantor, Stall: "CD43 (S7) expression identifies peripheral B cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 12, pp. 5503-15, 1995 (PubMed).

Rolink, ten Boekel, Melchers et al.: "A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 187-94, 1996 (PubMed).

Thurman, Walker, Jayaraman et al.: "Regulation of in vitro and in vivo T cell activation by CD43." in: International immunology, Vol. 10, Issue 5, pp. 691-701, 1998 (PubMed).

Woodman, Johnston, Hickey et al.: "The functional paradox of CD43 in leukocyte recruitment: a study using CD43-deficient mice." in: The Journal of experimental medicine, Vol. 188, Issue 11, pp. 2181-6, 1999 (PubMed).

Product: Stockton, Cheng, Manjunath et al.: "Negative regulation of T cell homing by CD43." in: Immunity, Vol. 8, Issue 3, pp. 373-81, 1998 (PubMed).