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CD19 Molecule (CD19) antibody

Details for Product No. ABIN967381
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Antigen
Synonyms CD19, AW495831, B4, CVID3
Reactivity
Mouse (Murine)
(423), (289), (46), (41), (41), (24), (24), (2)
Host
Rat
(357), (149), (147), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(77), (60), (60), (42), (15), (11), (10), (10), (9), (8), (8), (7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunoprecipitation (IP)
(464), (103), (89), (67), (58), (58), (49), (43), (27), (26), (14), (1), (1), (1)
Pubmed 12 references available
Quantity 0.5 mg
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Catalog No. ABIN967381
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Immunogen Mouse CD19 Transfected Cell Line
Clone 1D3
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD19
Background The 1D3 antibody reacts with CD19, a B lymphocyte-lineage differentiation antigen. CD19, a 95kDa transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. Terminally differentiated plasma cells do not express CD19. On the surface of mature B cells, the CD19 molecule associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with CD19-deficient mice have suggested that the level of CD19 expression affects the generation and maturation of B cells in the bone marrow and periphery. B-1 lineage B cells, also known as CD5+ B cells, are drastically reduced or absent in CD19-deficient mice. Increased levels of CD19 expression correlate with increased frequencies of peritonal and splenic B-1 cells and reduced numbers of conventional B lymphocytes in the periphery. CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD20/CD35, and it is proposed to play a role in complement-mediated mast-cell activation. This antibody is routinely tested by flow cytometric analysis.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-CD19 Molecule (CD19) antibody Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 and either purified rat IgG2a, kappa isotype control mAb R35-95 (first panel) or purified rat anti-mouse CD19 mAb 1D3 (second panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
anti-CD19 Molecule (CD19) antibody (2) Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 and either purified rat IgG2a, kappa isotype control mAb R35-95 (first panel) or purified rat anti-mouse CD19 mAb 1D3 (second panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
General Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, 1994 (PubMed).

Engel, Zhou, Ord et al.: "Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule." in: Immunity, Vol. 3, Issue 1, pp. 39-50, 1995 (PubMed).

Rickert, Rajewsky, Roes: "Impairment of T-cell-dependent B-cell responses and B-1 cell development in CD19-deficient mice." in: Nature, Vol. 376, Issue 6538, pp. 352-5, 1995 (PubMed).

Fearon: "The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes." in: Current opinion in immunology, Vol. 5, Issue 3, pp. 341-8, 1993 (PubMed).

Krop, de Fougerolles, Hardy et al.: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, 1996 (PubMed).

Krop, Shaffer, Fearon et al.: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, 1996 (PubMed).

Sato, Ono, Steeber et al.: "CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4371-8, 1996 (PubMed).

Sato, Steeber, Jansen et al.: "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4662-9, 1997 (PubMed).

Sato, Miller, Howard et al.: "Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 7, pp. 3278-87, 1997 (PubMed).

Sato, Jansen, Tedder: "CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 13158-62, 1998 (PubMed).

Inaoki, Sato, Weintraub et al.: "CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes." in: The Journal of experimental medicine, Vol. 186, Issue 11, pp. 1923-31, 1997 (PubMed).

Gommerman, Oh, Zhou et al.: "A role for CD21/CD35 and CD19 in responses to acute septic peritonitis: a potential mechanism for mast cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 6915-21, 2000 (PubMed).

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