CD19 Molecule (CD19) antibody

Antigen: CD19 Molecule (CD19)

Synonyms: B4, MGC12802, AW495831, SPNS1, MGC109570, CD19

Clonality: Monoclonal (1D3)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunoprecipitation (IP)

Catalog no.: ABIN967381

Additional Information

Alternative name: CD19

Immunogen: Mouse CD19 Transfected Cell Line

Format: Liquid

Isotype: IgG2a, kappa

Clone: 1D3

Description: The 1D3 antibody reacts with CD19, a B lymphocyte-lineage differentiation antigen. CD19, a 95kDa transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. Terminally differentiated plasma cells do not express CD19. On the surface of mature B cells, the CD19 molecule associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with CD19-deficient mice have suggested that the level of CD19 expression affects the generation and maturation of B cells in the bone marrow and periphery. B-1 lineage B cells, also known as CD5+ B cells, are drastically reduced or absent in CD19-deficient mice. Increased levels of CD19 expression correlate with increased frequencies of peritonal and splenic B-1 cells and reduced numbers of conventional B lymphocytes in the periphery. CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD20/CD35, and it is proposed to play a role in complement-mediated mast-cell activation. This antibody is routinely tested by flow cytometric analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 and either purified rat IgG2a, kappa isotype control mAb R35-95 (first panel) or purified rat anti-mouse CD19 mAb 1D3 (second panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 and either purified rat IgG2a, kappa isotype control mAb R35-95 (first panel) or purified rat anti-mouse CD19 mAb 1D3 (second panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Publications

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, 1994 (PubMed).

Engel, Zhou, Ord et al.: "Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule." in: Immunity, Vol. 3, Issue 1, pp. 39-50, 1995 (PubMed).

Rickert, Rajewsky, Roes: "Impairment of T-cell-dependent B-cell responses and B-1 cell development in CD19-deficient mice." in: Nature, Vol. 376, Issue 6538, pp. 352-5, 1995 (PubMed).

Fearon: "The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes." in: Current opinion in immunology, Vol. 5, Issue 3, pp. 341-8, 1993 (PubMed).

Krop, de Fougerolles, Hardy et al.: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, 1996 (PubMed).

Krop, Shaffer, Fearon et al.: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, 1996 (PubMed).

Sato, Ono, Steeber et al.: "CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4371-8, 1996 (PubMed).

Sato, Steeber, Jansen et al.: "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4662-9, 1997 (PubMed).

Sato, Miller, Howard et al.: "Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 7, pp. 3278-87, 1997 (PubMed).

Sato, Jansen, Tedder: "CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 13158-62, 1998 (PubMed).

Inaoki, Sato, Weintraub et al.: "CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes." in: The Journal of experimental medicine, Vol. 186, Issue 11, pp. 1923-31, 1997 (PubMed).

Gommerman, Oh, Zhou et al.: "A role for CD21/CD35 and CD19 in responses to acute septic peritonitis: a potential mechanism for mast cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 6915-21, 2000 (PubMed).