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Complement Receptor 2 (CR2) antibody

Details for Product No. ABIN967384, Supplier: Log in to see
  • Cd21
  • C3DR
  • CD21
  • CD35
  • Cr-1
  • Cr-2
  • Cr1
Mouse (Murine)
Clonality (Clone)
Monoclonal ()
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
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Brand BD Pharmingen™
Immunogen Purified Mouse CR1
Clone 7G6
Isotype IgG2b, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD21/CD35
Background The 7G6 antibody recognizes an epitope shared by 145-150-kDa and 190-kDa complement receptor proteins, originally designated CR2 (CD21) and CR1 (CD35), respectively. In the mouse, CD21 and CD35 are expressed on the majority of peripheral B lymphocytes, on the majority of resident peritoneal macrophages and mast cells, on peripheral blood granulocytes after treatment with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets. CD21 is a ligand-binding component of the CD19/CD21/CD81 signal-transduction complex associated with the antigen receptor on B lymphocytes. CD21/CD35 also co-localizes with CD19 on the surface of peritoneal mast cells. Cr2null mice display impaired inflammatory and humoral immune responses in vivo. The 7G6 mAb has been reported to inhibit rosette formation by C3d-bearing sheep erythrocytes, to block the complement dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD21/CD35 expression upon in vivo application, thus inhibiting primary antibody responses to immunization. Co-stimulation of B-cell differentiation via Sepharose-coupled 7G6 antibody has also been observed. The 7G6 mAb recognizes an epitope on CD35 distinct from the epitope recognized by anti-mouse CD35, clone 8C12 (ABIN967625, for the purified antibody), and it does not block binding of 8C12 mAb to mouse CD35. This antibody is routinely tested by flow cytometric analysis.
Synonyms: CR2/CR1
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Supplier Images
Flow Cytometry (FACS) image for anti-Complement Receptor 2 (CR2) antibody (ABIN967384) Expression of CD21/CD35 on splenic B lymphocytes. BALB/c splenocytes were stained wit...
Flow Cytometry (FACS) image for anti-Complement Receptor 2 (CR2) antibody (ABIN967384) Expression of CD21/CD35 on splenic B lymphocytes. BALB/c splenocytes were stained wit...
Product cited in: Cariappa, Tang, Parng et al.: "The follicular versus marginal zone B lymphocyte cell fate decision is regulated by Aiolos, Btk, and CD21." in: Immunity, Vol. 14, Issue 5, pp. 603-15, 2001 (PubMed).

Gommerman, Oh, Zhou et al.: "A role for CD21/CD35 and CD19 in responses to acute septic peritonitis: a potential mechanism for mast cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 6915-21, 2000 (PubMed).

Oliver, Martin, Kearney: "IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7198-207, 1999 (PubMed).

Fischer, Goerg, Shen et al.: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, 1998 (PubMed).

Oliver, Martin, Gartland et al.: "Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses." in: European journal of immunology, Vol. 27, Issue 9, pp. 2366-74, 1997 (PubMed).

Hu, Martin, Weis et al.: "Expression of the murine CD21 gene is regulated by promoter and intronic sequences." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4758-68, 1997 (PubMed).

Axcrona, Gray, Leanderson: "Regulation of B cell growth and differentiation via CD21 and CD40." in: European journal of immunology, Vol. 26, Issue 9, pp. 2203-7, 1996 (PubMed).

Ahearn, Fischer, Croix et al.: "Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen." in: Immunity, Vol. 4, Issue 3, pp. 251-62, 1996 (PubMed).

Molina, Holers, Li et al.: "Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3357-61, 1996 (PubMed).

Dempsey, Allison, Akkaraju et al.: "C3d of complement as a molecular adjuvant: bridging innate and acquired immunity." in: Science (New York, N.Y.), Vol. 271, Issue 5247, pp. 348-50, 1996 (PubMed).

Yoshida, van den Berg, Dijkstra: "Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping." in: Immunology, Vol. 80, Issue 1, pp. 34-9, 1993 (PubMed).

Martin, Weis: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, 1993 (PubMed).

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, 1994 (PubMed).

Kurtz, OToole, Christensen et al.: "The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 9, pp. 3581-91, 1990 (PubMed).

Thyphronitis, Kinoshita, Inoue et al.: "Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 224-30, 1991 (PubMed).

Wiersma, Kinoshita, Heyman: "Inhibition of immunological memory and T-independent humoral responses by monoclonal antibodies specific for murine complement receptors." in: European journal of immunology, Vol. 21, Issue 10, pp. 2501-6, 1991 (PubMed).

Kinoshita, Thyphronitis, Tsokos et al.: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, 1991 (PubMed).

Heyman, Wiersma, Kinoshita: "In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 665-8, 1990 (PubMed).

Background publications Cantarelli, Carissimi, Gentili et al.: "JAMA patient page: sexually transmitted diseases (chlamydia)." in: JAMA : the journal of the American Medical Association, Vol. 280, Issue 6, pp. 582, 1998 (PubMed).

Masuda, Makino, Kasajima: "Contribution of Thy-1-CD4+ T cells to the disorganization of lymphoid follicles in retrovirus-induced immunodeficiency syndrome, MAIDS." in: Clinical immunology and immunopathology, Vol. 81, Issue 3, pp. 253-60, 1997 (PubMed).

Kinoshita, Takeda, Hong et al.: "Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 9, pp. 3066-72, 1988 (PubMed).