Myogenic Differentiation 1 (MYOD1) antibody

Details for Product No. ABIN967403
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Antigen
Synonyms MyoD, XMyoD, XtmyoD, mf25, myf3, pum, MYOD1, MYOD, myod1, myod, MYF3, PUM, bHLHc1, AI503393, Myod-1, MUF3, etID309723.25, wu:fb57a01, zgc:136744
Reactivity
Mouse (Murine)
(108), (85), (62), (24), (24), (19), (12), (4), (1), (1)
Host
Mouse
(91), (45)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
(86), (42), (21), (20), (20), (16), (12), (11), (10), (10), (9), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN967403
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Immunogen Recombinant Mouse MyoD
Clone MoAb 5-8A
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MyoD
Background MyoD is a member of a family of myogenic basic helix-loop-helix (bHLH) transcription factors that includes Myf-5, myogenin, and MRF4 (also known as herculin or Myf-6). Members of this family, expressed exclusively in skeletal muscle, play a key role in myogenesis by activating muscle-specific genes in response to extracellular growth factors. Transfection of MyoD and other family members into non-muscle cells has been shown to either convert these cells to myogenic cells or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. Members of this family have distinct roles in muscle development, for example, MyoD and Myf-5 act early during myeloid development, whereas myogenin acts at a later point during myoblast differentiation. The expression and activity of MyoD has been shown to involve the mitogen-activated protein kinase (MAPK) cascade. This antibody is routinely tested by western blot analysis. The epitope for MoAb 5.8A has been mapped to amino acids 180-189nof mouse MyoD.
Molecular Weight 45/48 kDa
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Myogenic Differentiation 1 (MYOD1) antibody Western blot analysis of MyoD. Lysate from Rh30 rhabdomyosarcoma cells was probed with anti-MyoD (clone MoAb 5.8A, ABIN967403). MyoD is identified as an ~45 kDa band.
anti-Myogenic Differentiation 1 (MYOD1) antibody (2) anti-Myogenic Differentiation 1 (MYOD1) antibody (Image 2)
General Dias, Parham, Shapiro et al.: "Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility." in: Cancer research, Vol. 52, Issue 23, pp. 6431-9, 1992 (PubMed).

Davis, Weintraub, Lassar: "Expression of a single transfected cDNA converts fibroblasts to myoblasts." in: Cell, Vol. 51, Issue 6, pp. 987-1000, 1988 (PubMed).

Dias, Dilling, Houghton: "The molecular basis of skeletal muscle differentiation." in: Seminars in diagnostic pathology, Vol. 11, Issue 1, pp. 3-14, 1994 (PubMed).

Gredinger, Gerber, Tamir et al.: "Mitogen-activated protein kinase pathway is involved in the differentiation of muscle cells." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10436-44, 1998 (PubMed).

Rawls, Valdez, Zhang et al.: "Overlapping functions of the myogenic bHLH genes MRF4 and MyoD revealed in double mutant mice." in: Development (Cambridge, England), Vol. 125, Issue 13, pp. 2349-58, 1998 (PubMed).

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