Myogenic Differentiation 1 (MYOD1) antibody

Antigen: Myogenic Differentiation 1 (MYOD1)

Synonyms: PUM, MYF3, MYOD, bHLHc1, MyoD, MGC143434, pum, myf3, XMyoD, XtmyoD, MGC75960, MUF3, Myod-1, AI503393, myod, MGC136744, wu:fb57a01, zgc:136744, etID309723.25, MGC156574, MYOD1, myod1

Clonality: Monoclonal (MoAb 5-8A)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN967403

Additional Information

Alternative name: MyoD

Immunogen: Recombinant Mouse MyoD

Cross-Reactivity: Human, Rat (Rattus), Chicken

Format: Liquid

Isotype: IgG1

Clone: MoAb 5-8A

Description: MyoD is a member of a family of myogenic basic helix-loop-helix (bHLH) transcription factors that includes Myf-5, myogenin, and MRF4 (also known as herculin or Myf-6). Members of this family, expressed exclusively in skeletal muscle, play a key role in myogenesis by activating muscle-specific genes in response to extracellular growth factors. Transfection of MyoD and other family members into non-muscle cells has been shown to either convert these cells to myogenic cells or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. Members of this family have distinct roles in muscle development, for example, MyoD and Myf-5 act early during myeloid development, whereas myogenin acts at a later point during myoblast differentiation. The expression and activity of MyoD has been shown to involve the mitogen-activated protein kinase (MAPK) cascade. This antibody is routinely tested by western blot analysis. The epitope for MoAb 5.8A has been mapped to amino acids 180-189nof mouse MyoD.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 45/48 kDa

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Restrictions: For Research Use only

Images

Western blot analysis of MyoD. Lysate from Rh30 rhabdomyosarcoma cells was probed with anti-MyoD (clone MoAb 5.8A, ABIN967403). MyoD is identified as an ~45 kDa band.

anti-Myogenic Differentiation 1 (MYOD1) antibody (Image 2)

Publications

Dias, Parham, Shapiro et al.: "Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility." in: Cancer research, Vol. 52, Issue 23, pp. 6431-9, 1992 (PubMed).

Davis, Weintraub, Lassar: "Expression of a single transfected cDNA converts fibroblasts to myoblasts." in: Cell, Vol. 51, Issue 6, pp. 987-1000, 1988 (PubMed).

Dias, Dilling, Houghton: "The molecular basis of skeletal muscle differentiation." in: Seminars in diagnostic pathology, Vol. 11, Issue 1, pp. 3-14, 1994 (PubMed).

Gredinger, Gerber, Tamir et al.: "Mitogen-activated protein kinase pathway is involved in the differentiation of muscle cells." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10436-44, 1998 (PubMed).

Rawls, Valdez, Zhang et al.: "Overlapping functions of the myogenic bHLH genes MRF4 and MyoD revealed in double mutant mice." in: Development (Cambridge, England), Vol. 125, Issue 13, pp. 2349-58, 1998 (PubMed).