Recombinant MYOD1 antibody

Catalog No. ABIN6940161

This Rabbit Monoclonal antibody specifically detects MYOD1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Hamster and Rabbit.

Quick Overview for Recombinant MYOD1 antibody (ABIN6940161)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster, Rabbit

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: MYOD1-2075R

Product Details anti-MYOD1 Antibody

Purpose: Recombinant MyoD1 (Rhabdomyosarcoma Marker) Antibody

Specificity: Recognizes a phospho-protein of 45 kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Purification: 200ug/ml of Ab purified by Protein A Column.

Immunogen: Recombinant human full-length MyoD1 protein

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: Fetal skeletal muscle or Rhabdomyosarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MYOD1

Background:

bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Human skeletal muscle or rhabdomyosarcoma, Heart

Molecular Weight: 45kDa

Gene ID: 4654, 181768

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development