Interleukin 4 (IL4) antibody

Details for Product No. ABIN967463
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Antigen
Synonyms IL4, IL-4, BCGF-1, BCGF1, BSF-1, BSF1, Il-4, Il4e12, IL2
Reactivity
Human
(212), (125), (35), (13), (7), (6), (4), (2), (2), (2), (2), (1), (1)
Host
Rat
(148), (118), (104), (6), (3), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(50), (19), (12), (10), (7), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Flow Cytometry (FACS), Western Blotting (WB)
(234), (204), (101), (48), (32), (30), (24), (23), (21), (21), (20), (20), (18), (8), (8), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN967463
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Immunogen Purified Recombinant Human IL-4
Clone MP4-25D2
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IL-4
Background The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described. This antibody is routinely tested as a blocking control for intracellular staining.
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Application Notes 1. Blocking Control for Intracellular Staining:
The unlabeled MP4-25D2 antibody can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated MP4-25D2 antibody. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled MP4-25D2 antibody (ABIN967463) for 20 minutes at 4°C, prior to staining with conjugated MP4-25D2 antibody. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

2. Neutralization:
The NA/LE™ MP4-25D2 antibody is useful for neutralization of human IL-4 bioactivity. A suitable NA/LE ™ rat IgG1 isotype control to match the MP4-25D2 antibody is the R3-34 antibody.

3. WB: The purified MP4-25D2 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Interleukin 4 (IL4) antibody Expression of IL-4 by stimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with soluble anti-human CD3 antibody (1 µg/ml), recombinant human IL-2 (10 ng/ml) and IL-4 (20 ng/ml) for 2 days. The cells were subsequently cultured in medium containing recombinant human IL-2 and recombinant human IL-4 for 3 days. Finally, the cells were harvested and stimulated for 6 h with PMA (Sigma) and calcium ionophore A23187 (Sigma) in the presence of 2 µM GolgiStop™. The cells were harvested, stained with PE-Cy™5 -anti CD4, fixed, permeabilized, and subsequently stained with 0.05 µg of PE-rat anti-human IL-4 antibody (PE-MP4-25D2) by using Pharmingen's staining protocol (first panel). To demonstrate specificity of staining, the binding of PE-MP4-25D2 antibody was blocked by preincubation of the fixed/permeabilized cells with unlabeled MP4-25D2 antibody (2.5 µg, ABIN967463, second panel) prior to staining. The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using unlabeled antibody blocking controls.
anti-Interleukin 4 (IL4) antibody (2) The binding of PE-MP4-25D2 antibody was blocked by preincubation of the fixed/permeabilized cells with unlabeled MP4-25D2 antibody (2.5 µg, ABIN967463, second panel) prior to staining.
General Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

Chrétien, Van Kimmenade, Pearce et al.: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989 (PubMed).

Jung, Schauer, Rieger et al.: "Interleukin-4 and interleukin-5 are rarely co-expressed by human T cells." in: European journal of immunology, Vol. 25, Issue 8, pp. 2413-6, 1995 (PubMed).

Ramanathan, Ingram, Sullivan et al.: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

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