HLA-DR-gamma (CD74) antibody
|Synonyms||DHLAG, HLADG, Ia-GAMMA, Ii, CLIP|
Alternatives Intracellular Flow Cytometry (ICFC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|12 references available|
|Quantity||0.5 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||A.TL mouse lymphocytes and B10.A mouse-derived CH1 B-cell lymphoma|
The In-1 antibody reacts with CD74, the MHC class II-associated Invariant chain (Ii), expressed in I-A+ cell populations. Although it is predominantly expressed intracellularly, it has also been detected on the cell surface of some B-cell lines and at low levels on a small number of B lymphocytes. In the mouse, alternatively spliced transcripts encode 31-kDa and 41-kDa isoforms, and the cell-surface CD74 has a chondroitin sulfate side chain which allows it to interact with CD44. CD74 is directly involved in the intracellular transport of MHC class II molecules and antigen presentation, and it affects the maturation of T and B lymphocytes.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Research Area||Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)|
|Restrictions||For Research Use only|
Mehringer, Harris, Kindle et al.: "Characterization of fragments of the murine Ia-associated invariant chain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 3, pp. 920-7, 1991 (PubMed).
Koch, Koch, Hämmerling: "Ia invariant chain detected on lymphocyte surfaces by monoclonal antibody." in: Nature, Vol. 299, Issue 5884, pp. 644-5, 1982 (PubMed).
Bodmer, Viville, Benoist et al.: "Diversity of endogenous epitopes bound to MHC class II molecules limited by invariant chain." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1284-6, 1994 (PubMed).
Viville, Neefjes, Lotteau et al.: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, 1993 (PubMed).
Cresswell: "Assembly, transport, and function of MHC class II molecules." in: Annual review of immunology, Vol. 12, pp. 259-93, 1994 (PubMed).
Bikoff, Huang, Episkopou et al.: "Defective major histocompatibility complex class II assembly, transport, peptide acquisition, and CD4+ T cell selection in mice lacking invariant chain expression." in: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1699-712, 1993 (PubMed).
Elliott, Drake, Amigorena et al.: "The invariant chain is required for intracellular transport and function of major histocompatibility complex class II molecules." in: The Journal of experimental medicine, Vol. 179, Issue 2, pp. 681-94, 1994 (PubMed).
Naujokas, Morin, Anderson et al.: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." in: Cell, Vol. 74, Issue 2, pp. 257-68, 1993 (PubMed).
Shachar, Flavell: "Requirement for invariant chain in B cell maturation and function." in: Science (New York, N.Y.), Vol. 274, Issue 5284, pp. 106-8, 1996 (PubMed).
Bertolino, Rabourdin-Combe: "The MHC class II-associated invariant chain: a molecule with multiple roles in MHC class II biosynthesis and antigen presentation to CD4+ T cells." in: Critical reviews in immunology, Vol. 16, Issue 4, pp. 359-79, 1997 (PubMed).
Farr, DeRoos, Eastman et al.: "Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells." in: European journal of immunology, Vol. 26, Issue 12, pp. 3185-93, 1997 (PubMed).
Nakagawa, Roth, Wong et al.: "Cathepsin L: critical role in Ii degradation and CD4 T cell selection in the thymus." in: Science (New York, N.Y.), Vol. 280, Issue 5362, pp. 450-3, 1998 (PubMed).
|Hosts||Mouse (111), Rat (8)|
|Reactivities||Human (109), Dog (Canine) (2), Primate (2), Rat (Rattus) (1)|
|Applications||Flow Cytometry (FACS) (95), Immunoprecipitation (IP) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Western Blotting (WB) (30), Immunocytochemistry (ICC) (14), Immunofluorescence (IF) (11), Immunohistochemistry (Fixed) (IHC (fx)) (9), Immunohistochemistry (IHC) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Functional Studies (Func) (2), Immunochromatography (IC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)|
|Conjugates||FITC (14), PE (8), Biotin (7), APC (5), Alexa Fluor 488 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), PE-Dyomics 590 (2), PE-Dyomics 647 (2), PerCP (2), RPE (2), PerCP-Cy5.5 (1)|
|Epitopes||AA 12-28 (3), C-Term (3)|