Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody
Alternatives Western Blotting (WB), ELISA (Capture)
|2 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Recombinant Human Fas|
Programmed cell death (apoptosis) may be induced in response to a variety of cytotoxic stimuli, including activation of surface receptors. An area of particular interest has been the induction of apoptosis by the receptor-ligand pair: Fas (CD95) and Fas Ligand (FasL). Fas is an ~45 kD cell surface protein which belongs to the the TNF (tumor necrosis factor receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL, a member of the TNF ligand family, is expressed on activated T and NK cells. FasL initiates signaling at the cell surface by aggregation of individual Fas recpetors via binding to the multivalent ligand. Antibodies which selectively activate Fas can be used in vitro to mimic the apoptotic response associated with FasL. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. Clone G254-274 recognizes human Fas. A soluble form of recombinant human Fas (lacking the Fas transmembrane domain) was used as immunogen.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||45 kDa|
Related Products: ABIN967460, ABIN967389
|Synonyms||APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, LOC574159, fasl, zgc:162027, FAS|
|Application Notes||Applications include western blot analysis (1-2µg/ml). Clone G254-274 has also been tested in antibody development for ELISA where G254-274 has been used as a capture antibody paired with the biotin mouse anti-human Fas clone DX2 (MN 555672) as the detection antibody. Clone G254-274 is not suggested for flow cytometric analysis or for use in functional apoptosis assays.|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Research Area||Immunology, Innate Immunity, Cytokines, CD Antigens, Apoptosis/Necrosis, Death Receptors, Cancer, Growth Factors, Surface Receptors of Immune Cells|
|Restrictions||For Research Use only|
|Western blot analysis of recombinant FasÄTM. Purified mouse anti-human Fas antibody (clone G254-274) was used to stain 200 ng of recombinant FasÄTM (MN ABIN967460). anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody (Image 2)|
Takahashi, Tanaka, Brannan et al.: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994 (PubMed).
Tanaka, Suda, Takahashi et al.: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, 1995 (PubMed).