Spleen tyrosine Kinase (SYK) (pTyr348) antibody

Details for Product No. ABIN967580
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Antigen
Synonyms p72-Syk, Sykb, p72syk
Epitope
pTyr348
(43), (29), (24), (19), (12), (9), (8), (8), (7), (7), (7), (7), (6), (6), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(267), (110), (103), (37), (27), (25), (12), (12), (3), (1)
Host
Mouse
(213), (52), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Flow Cytometry (ICFC)
(214), (101), (95), (76), (47), (45), (30), (29), (3), (2), (1), (1)
Pubmed 7 references available
Quantity 50 μg
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Catalog No. ABIN967580
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Immunogen Phosphorylated Human Syk Peptide
Clone I120-722
Isotype IgG1, kappa
Cross-Reactivity (Details) Predicted: Mouse, Rat
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Syk
Background Syk is a non-receptor protein-tyrosine kinase that is closely related to ZAP70 and plays crucial roles in the development and receptor-mediated signaling of most leukocytes and in vascular integrity. Syk is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. Mature (CD4 or CD8 single-positive) thymocytes and peripheral alphabeta TCR-bearing T lymphocytes have very low or undetectable levels of Syk. Syk contributes to the signal transduction process by binding to ITAMs (Immunoreceptor Tyrosine-based Activation Motifs) of immune receptors, including Igalpha and Igbeta (CD79a and b), TCRzeta, CD3epsilon, and FcRgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in the interdomain, between the SH2 and Kinase domains of Syk, to undergo auto-phosphorylation and phosphorylation by Lyn. The tyrosine 348 phosphorylation site (pY348) in human Syk is orthologous to tyrosine 342 in mouse and rat Syk and tyrosine 315 in human ZAP70. This phosphorylated site can act as a binding site for other signaling molecules, such as PLCgamma, Vav, and Fgr. The I120-772 antibody is specific for human Syk (pY348) and does not cross-react with phosphorylated Zap70. The orthologous phosphorylation site in mouse and rat Syk is Y342.
Molecular Weight 72 kDa
Research Area Immunology, Innate Immunity, Tyrosine Kinases, Signaling
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Spleen tyrosine Kinase (SYK) (pTyr348) antibody Lysate from control (First Panel) and pervanadate-treated (Second Panel) Ramos cells (Burkitt's lymphoma) were probed with mAb I120-722 at concentrations of 0.25 (lanes 1), 0.125 (lanes 2), and 0.0625 (lanes 3) µg/ml. Syk (pY348) is identified as a strong band of 72 kDa in the pervanadate-treated Ramos cells.
anti-Spleen tyrosine Kinase (SYK) (pTyr348) antibody (2) Syk (pY348) staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadate solution for 2 hours (top row) or unstimulated (bottom row), was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with Purified Mouse anti-Syk (pY348) monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X.
Product cited in: Turner, Schweighoffer, Colucci et al.: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

Coopman, Do, Barth et al.: "The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells." in: Nature, Vol. 406, Issue 6797, pp. 742-7, 2000 (PubMed).

Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, 2001 (PubMed).

Vines, Potter, Xu et al.: "Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr." in: Immunity, Vol. 15, Issue 4, pp. 507-19, 2001 (PubMed).

Hong, Yankee, Harrison et al.: "Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. A mechanism for negative signaling by the Lyn tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 31703-14, 2002 (PubMed).

Zhang, Berenstein, Siraganian: "Phosphorylation of Tyr342 in the linker region of Syk is critical for Fc epsilon RI signaling in mast cells." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8144-54, 2002 (PubMed).

Abtahian, Guerriero, Sebzda et al.: "Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk." in: Science (New York, N.Y.), Vol. 299, Issue 5604, pp. 247-51, 2003 (PubMed).

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