Spleen tyrosine Kinase (SYK) (pTyr348) antibody

Antigen: Spleen tyrosine Kinase (SYK)

Synonyms: FLJ25043, FLJ37489, DKFZp313N1010, Syk, p72syk

Binding Site: pTyr348

Clonality: Monoclonal (I120-722)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN967580

Additional Information

Alternative name: Syk

Immunogen: Phosphorylated Human Syk Peptide

Cross-Reactivity (Details): Predicted: Mouse, Rat

Format: Liquid

Isotype: IgG1, kappa

Clone: I120-722

Description: Syk is a non-receptor protein-tyrosine kinase that is closely related to ZAP70 and plays crucial roles in the development and receptor-mediated signaling of most leukocytes and in vascular integrity. Syk is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. Mature (CD4 or CD8 single-positive) thymocytes and peripheral alphabeta TCR-bearing T lymphocytes have very low or undetectable levels of Syk. Syk contributes to the signal transduction process by binding to ITAMs (Immunoreceptor Tyrosine-based Activation Motifs) of immune receptors, including Igalpha and Igbeta (CD79a and b), TCRzeta, CD3epsilon, and FcRgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in the interdomain, between the SH2 and Kinase domains of Syk, to undergo auto-phosphorylation and phosphorylation by Lyn. The tyrosine 348 phosphorylation site (pY348) in human Syk is orthologous to tyrosine 342 in mouse and rat Syk and tyrosine 315 in human ZAP70. This phosphorylated site can act as a binding site for other signaling molecules, such as PLCgamma, Vav, and Fgr. The I120-772 antibody is specific for human Syk (pY348) and does not cross-react with phosphorylated Zap70. The orthologous phosphorylation site in mouse and rat Syk is Y342.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 72 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Immunology, Innate Immunity, Tyrosine Kinases, Signaling

Restrictions: For Research Use only

Images

Lysate from control (First Panel) and pervanadate-treated (Second Panel) Ramos cells (Burkitt's lymphoma) were probed with mAb I120-722 at concentrations of 0.25 (lanes 1), 0.125 (lanes 2), and 0.0625 (lanes 3) µg/ml. Syk (pY348) is identified as a strong band of 72 kDa in the pervanadate-treated Ramos cells.

Syk (pY348) staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadate solution for 2 hours (top row) or unstimulated (bottom row), was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with Purified Mouse anti-Syk (pY348) monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X.

Publications

Product: Turner, Schweighoffer, Colucci et al.: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

Coopman, Do, Barth et al.: "The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells." in: Nature, Vol. 406, Issue 6797, pp. 742-7, 2000 (PubMed).

Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, 2001 (PubMed).

Vines, Potter, Xu et al.: "Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr." in: Immunity, Vol. 15, Issue 4, pp. 507-19, 2001 (PubMed).

Hong, Yankee, Harrison et al.: "Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. A mechanism for negative signaling by the Lyn tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 31703-14, 2002 (PubMed).

Zhang, Berenstein, Siraganian: "Phosphorylation of Tyr342 in the linker region of Syk is critical for Fc epsilon RI signaling in mast cells." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8144-54, 2002 (PubMed).

Abtahian, Guerriero, Sebzda et al.: "Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk." in: Science (New York, N.Y.), Vol. 299, Issue 5604, pp. 247-51, 2003 (PubMed).