Spleen tyrosine Kinase (SYK) (AA 5-360) antibody

Details for Product No. ABIN94475
Antigen
Epitope
AA 5-360
59
48
26
22
19
19
17
15
12
11
11
11
10
9
8
8
7
6
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
408
182
167
24
9
8
8
5
3
3
1
Host
Mouse
332
71
9
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
15
10
9
7
7
7
7
7
7
6
6
6
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
315
172
166
75
66
48
31
27
12
10
4
4
3
1
1
1
Options
Immunogen Recombinant fragment (aa 5-360) of human Syk.
Clone SYK-01
Isotype IgG1
Specificity The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk, Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).
Cross-Reactivity (Details) Mouse, Human, Rat, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Syk (SYK Antibody Abstract)
Background Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Pathways Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Spleen tyrosine Kinase (SYK) (AA 5-360) antibody (ABIN94475) Immunofluorescence staining of Syk in human primary fibroblasts using anti-Syk (; gre...
Image no. 2 for anti-Spleen tyrosine Kinase (SYK) (AA 5-360) antibody (ABIN94475) Immunofluorescence staining of Syk in human HeLa cell line using anti-Syk
Image no. 3 for anti-Spleen tyrosine Kinase (SYK) (AA 5-360) antibody (ABIN94475) Western Blotting analysis (non-reducing conditions) of whole cell lysate of RAMOS hum...
Product cited in: Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, 2006 (PubMed).

Hálová, Dráberová, Dráber: "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor." in: International immunology, Vol. 14, Issue 2, pp. 213-23, 2002 (PubMed).

Arudchandran, Brown, Peirce, Song, Zhang, Siraganian, Blank, Rivera: "The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1." in: The Journal of experimental medicine, Vol. 191, Issue 1, pp. 47-60, 2000 (PubMed).