Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr249) antibody

Details for Product No. ABIN967617
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Antigen
Synonyms MGC68466, cas, Crkas, p130cas, BCAR1, fc17f11, wu:fc17f11, zgc:175192, CAS, CAS1, CASS1, CRKAS, P130Cas, AI385681, Cas, P130CAS
Epitope
pTyr249
(31), (18), (17), (17), (12), (6), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(161), (90), (89), (16), (12), (4), (1), (1)
Host
Mouse
(146), (21), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB)
(115), (56), (50), (47), (34), (17), (16), (7), (3), (3)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN967617
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Immunogen Phosphorylated Human p130Cas
Clone J169-757-12-2
Isotype IgG2b, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p130 Cas
Background P47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule.
The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.
Molecular Weight 130 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr249) antibody Western blot analysis of p130Cas (pY249) in human Burkitt’s lymphoma. Lysates from control (first panel) and hydrogen peroxide-activated (second panel) Ramos cells were probed with purified mouse anti-p130Cas (pY249) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.0312 µg/ml (lanes 3 and 6). p130Cas (pY249) is identified as a band of 130 kDa in the treated cells.
Product cited in: Hinsby, Olsen, Bennett et al.: "Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 2, Issue 1, pp. 29-36, 2003 (PubMed).

Goldberg, Alexander, Pellicena et al.: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, 2003 (PubMed).

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