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CD44 (Pgp-1, H-CAM, Ly24) antibody

Details for Product No. ABIN967622, Supplier: Log in to see
Mouse (Murine)
Clonality (Clone)
Monoclonal ()
Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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Brand BD Pharmingen™
Immunogen (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell clone BMS2
Clone KM114
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background The KM114 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The KM114 hybridoma was produced at the same time as the published clone KM201, and the mAb recognizes a different epitope from that recognized by mAb IM7. The standard form of CD44, lacking variable exons and referred to as CD44H or CD44S, is widely expressed on hematopoietic and non-hematopoietic cells. CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have lower expression of CD44H on T cells. CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1).10 Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44. In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells, and memory cells can be recognized by their CD44[hi] phenotype. KM114 antibody can be used in ELISA to detect soluble CD44, and it is effective for in vitro blocking of hyaluronate recognition by CD44. It has been reported that KM114 mAb cross-reacts with CD44 of the Chinese hamster, Cricetulus griseus, but not human CD44.
Synonyms: Pgp-1, H-CAM, Ly-24
Application Notes For quantitation of soluble CD44, a sandwich ELISA using purified IM7 antibody for capture and biotinylated KM114 mAb for detection has been described. For IHC, we recommend the use of purified IM7 mAb in our special formulation for immunohistochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Supplier Images
Flow Cytometry (FACS) image for anti-CD44 (Pgp-1, H-CAM, Ly24) antibody (ABIN967622) Blocking of hyaluronate binding by KM114 antibody. The BALB/c B-cell hybridoma BM-2 w...
Product cited in: Castro, Bono, Simon, Vargas, Rosemblatt: "Spleen-derived stromal cells. Adhesion molecules expression and lymphocyte adhesion to reticular cells." in: European journal of cell biology, Vol. 74, Issue 4, pp. 321-8, 1998

Naor, Sionov, Ish-Shalom: "CD44: structure, function, and association with the malignant process." in: Advances in cancer research, Vol. 71, Issue 4, pp. 241-319, 1997

Weber, Ashkar, Glimcher, Cantor: "Receptor-ligand interaction between CD44 and osteopontin (Eta-1)." in: Science (New York, N.Y.), Vol. 271, Issue 5248, pp. 509-12, 1996

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, 1995

Katoh, Zheng, Oritani, Shimozato, Kincade: "Glycosylation of CD44 negatively regulates its recognition of hyaluronan." in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 419-29, 1995

Zheng, Katoh, He, Oritani, Miyake, Lesley, Hyman, Hamik, Parkhouse, Farr, Kincade: "Monoclonal antibodies to CD44 and their influence on hyaluronan recognition." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 485-95, 1995

Katoh, McCarthy, Kincade: "Characterization of soluble CD44 in the circulation of mice. Levels are affected by immune activity and tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3440-9, 1994

Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, 1994

Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." in: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, 1993

Naujokas, Morin, Anderson, Peterson, Miller: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." in: Cell, Vol. 74, Issue 2, pp. 257-68, 1993

Murakami, Miyake, June, Kincade, Hodes: "IL-5 induces a Pgp-1 (CD44) bright B cell subpopulation that is highly enriched in proliferative and Ig secretory activity and binds to hyaluronate." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3618-27, 1991

MacDonald, Budd, Cerottini: "Pgp-1 (Ly 24) as a marker of murine memory T lymphocytes." in: Current topics in microbiology and immunology, Vol. 159, Issue 4, pp. 97-109, 1990

Miyake, Medina, Hayashi, Ono, Hamaoka, Kincade: "Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 477-88, 1990

Lynch, Ceredig: "Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation." in: European journal of immunology, Vol. 19, Issue 2, pp. 223-9, 1989

Background publications Kanamori, Ishimaru, Nanno, Maki, Ikuta, Nariuchi, Ishikawa: "Identification of novel lymphoid tissues in murine intestinal mucosa where clusters of c-kit+ IL-7R+ Thy1+ lympho-hemopoietic progenitors develop." in: The Journal of experimental medicine, Vol. 184, Issue 4, pp. 1449-59, 1996

Godfrey, Kennedy, Suda, Zlotnik: "A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4244-52, 1993