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CR1 antibody (Complement Component Receptor 1 (CD35))

Details for Product anti-CR1 Antibody No. ABIN967625, Supplier: Log in to see
Antigen
  • C3BR
  • C3DR
  • C4BR
  • CD21
  • CD35
  • Cr-1
  • Cr-2
  • Cr1
  • KN
Alternatives
anti-Mouse (Murine) CR1 antibody for Blocking Reagent
Reactivity
Mouse (Murine)
194
17
14
10
10
5
5
2
Host
Rat
168
28
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CR1 antibody is un-conjugated
33
30
10
3
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
148
75
60
42
42
37
21
20
11
11
11
4
4
1
1
1
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Purified mouse CR1
Clone 8C12
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD35 (CR1) (CR1 Antibody Abstract)
Background The 8C12 antibody recognizes an epitope present on the 190-kDa complement receptor protein, originally designated CR1 (CD35), but not the 145-150-kDa CR2 (CD21) molecule. Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane proteins resulting from the alternative splicing of mRNA transcribed from the Cr2 gene. Therefore, an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a), respectively. The epitope recognized by 8C12 mAb is only present on CD35/CD21b. Moreover, it has also been proposed that Crry is the true mouse genetic homologue of human CR1 (CD35). In the mouse, CD35 is expressed on the majority of peripheral B cells, on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatment with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets. In addition, it has not been detected, at the protein or mRNA level, in the macrophage cell line J774, bone marrow-derived macrophages, or thioglycollate-elicited peritoneal macrophages. The 8C12 mAb has been reported to inhibit rosette formation by C3bbearing sheep erythrocytes, to block the complement-dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD35 expression upon in vivo application, inhibiting only some primary antibody responses to immunization. B lymphocytes of Cr2[null] mice display impaired humoral immune responses in vivo. The 8C12 mAb recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/CD35 mAb 7G6 (ABIN967384), and it does not block binding by 7G6 mAb to CD35. *Please note that the isotype of 8C12 mAb was originally reported to be Rat IgG2c. Further investigations have demonstrated that the isotype of 8C12 mAb is Rat IgG2a.
Synonyms: CR1, CD21b
Pathways Complement System
Comment

Related Products: ABIN967384

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Product cited in: Fischer, Goerg, Shen, Prodeus, Goodnow, Kelsoe, Carroll: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, 1998

Hu, Martin, Weis, Weis: "Expression of the murine CD21 gene is regulated by promoter and intronic sequences." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4758-68, 1997

Molina, Holers, Li, Fung, Mariathasan, Goellner, Strauss-Schoenberger, Karr, Chaplin: "Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3357-61, 1996

Ahearn, Fischer, Croix, Goerg, Ma, Xia, Zhou, Howard, Rothstein, Carroll: "Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen." in: Immunity, Vol. 4, Issue 3, pp. 251-62, 1996

Martin, Weis: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, 1993

Yoshida, van den Berg, Dijkstra: "Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping." in: Immunology, Vol. 80, Issue 1, pp. 34-9, 1993

Kinoshita, Thyphronitis, Tsokos, Finkelman, Hong, Sakai, Inoue: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, 1991

Heyman, Wiersma, Kinoshita: "In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 665-8, 1990

Kurtz, OToole, Christensen, Weis: "The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 9, pp. 3581-91, 1990

Background publications Wiersma, Kinoshita, Heyman: "Inhibition of immunological memory and T-independent humoral responses by monoclonal antibodies specific for murine complement receptors." in: European journal of immunology, Vol. 21, Issue 10, pp. 2501-6, 1991

Kinoshita, Takeda, Hong, Kozono, Sakai, Inoue: "Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 9, pp. 3066-72, 1988