CD35 antibody

Catalog No. ABIN2749062

There is 1 publication for this product available. The Mouse Monoclonal anti-CD35 antibody is suitable to detect CD35 in samples from Human and Non-Human Primate. It has been validated for FACS, IHC (p), IP, IHC (fro) and ICC.

Quick Overview for CD35 antibody (ABIN2749062)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: E11

Product Details anti-CD35 Antibody

Purpose: Anti-Hu CD35 Purified

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The mouse monoclonal antibody E11 recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Acute monocytic leukemia cells and normal blood monocytes

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval.
Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD35 antibody (ABIN2749062)

Hogg, Ross, Jones, Slusarenko, Walport, Lachmann: "Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1)." in: European journal of immunology, Vol. 14, Issue 3, pp. 236-43, (1984) (PubMed).

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35

Background: Complement C3b/C4b receptor 1 (Knops blood group),CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.,CR1, KN, C3BR, C4BR

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity