Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (C-Term) antibody

Details for Product No. ABIN967637
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Synonyms AW556805, Mcl-1, BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, TM, bcl2-L-3, mcl1/EAT, mcl-1
(28), (27), (20), (15), (14), (12), (12), (10), (10), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(291), (86), (61), (8), (4), (4), (4), (3), (2), (1), (1), (1)
(201), (74), (17), (2)
Clonality (Clone)
Monoclonal ()
(10), (7), (7), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB), Immunoprecipitation (IP)
(251), (114), (96), (74), (57), (39), (27), (25), (20), (11), (10), (6), (6), (5), (1), (1), (1)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN967637
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Immunogen Purified recombinant human Mcl-1 lacking the hydrophobic domain
Clone LPR-02
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Mcl-1
Background The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Mcl-1 is a member of the anti-apoptotic Bcl-2 group of proteins, including Bcl-x, Bcl-w, A1/Bfl-1, Boo/Diva, and Nr-13. Mcl-1 was cloned from the ML-1 human myeoblastic leukemia cell line. Mcl-1 localizes to the mitochrondria, like Bcl-2, but also associates with membranes via its C-terminal hydrophobic domain. Further studies have shown that Mcl-1 is upregulated in response to the GM-CSF and IL-3 cytokine signaling pathways and that this regulation of Mcl-1 is controlled at the level of transcription. Mcl-1 is detected at approximately 40 kDa in SDS/PAGE.
Molecular Weight 40 kDa
Research Area Cancer, Apoptosis/Necrosis
Application Notes The antibody is tested by Western blot on K-562 cell lysate (ATCC CCL-243). Other applications include immunoprecipitation (1-2 µg/ml).

Related Products: ABIN968586, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (C-Term) antibody Western blot analysis of Mcl-1. Lysates from K-562 cells were probed with anti-human Mcl-1 antibody (clone 22, ABIN967637) at a concentration of 2 myg/ml. Mcl-1 is identified at approximately 40 kDa.
Product cited in: Wang, Chao, Chen et al.: "The antiapoptotic gene mcl-1 is up-regulated by the phosphatidylinositol 3-kinase/Akt signaling pathway through a transcription factor complex containing CREB." in: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6195-206, 1999 (PubMed).

Gross, McDonnell, Korsmeyer: "BCL-2 family members and the mitochondria in apoptosis." in: Genes & development, Vol. 13, Issue 15, pp. 1899-911, 1999 (PubMed).

Adams, Cory: "The Bcl-2 protein family: arbiters of cell survival." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, 1998 (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).

Yang, Kozopas, Craig: "The intracellular distribution and pattern of expression of Mcl-1 overlap with, but are not identical to, those of Bcl-2." in: The Journal of cell biology, Vol. 128, Issue 6, pp. 1173-84, 1995 (PubMed).

Kozopas, Yang, Buchan et al.: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, 1993 (PubMed).

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