RAS (AA 1-190) antibody

Antigen: RAS

Binding Site: AA 1-190

Clonality: Monoclonal (18)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967686

Additional Information

Immunogen: Human Ras (Ha-ras)

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 18

Description: Ras and related proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB ) which, upon mutational activation, can function as independent oncogenes. These proteins relay signals from tyrosine kinases at the plasma membrane which subsequently lead to the nucleus via a network of serine/threonine kinases . The p21ras protein is active in its GTP-bound state. This form is slowly converted to the GDP-bound form by the intrinsic GTPase activity of Ras. This activity is greatly enhanced byGTPase-activating proteins (GAPs) which subsequently lead to removal of the GTP molecule and replacement with GDP. Maintenance of Ras in the GTP form can lead to transformation. One class of Ras mutations, commonly found in human tumors, results in an accumulation of Ras-GTP. The mutant Ras can bind GAP, but GAP bound in this manner seems unable to affect the Ras-GTPase activity. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 21 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cancer, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of Ras on A431 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Ras antibody.

Immunofluorescent staining of Hs 766T cells.

Publications

Dhillon, Pollock, Steen et al.: "Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3237-46, 2002 (PubMed).

Product: Bourne, Sanders, McCormick: "The GTPase superfamily: a conserved switch for diverse cell functions." in: Nature, Vol. 348, Issue 6297, pp. 125-32, 1990 (PubMed).

Gibbs, Marshall: "The ras oncogene--an important regulatory element in lower eucaryotic organisms." in: Microbiological reviews, Vol. 53, Issue 2, pp. 171-85, 1989 (PubMed).

Spaargaren, Bos: "Rab5 induces Rac-independent lamellipodia formation and cell migration." in: Molecular biology of the cell, Vol. 10, Issue 10, pp. 3239-50, 1999 (PubMed).

Garcia, de Gunzburg, Eychène et al.: "Thrombopoietin-mediated sustained activation of extracellular signal-regulated kinase in UT7-Mpl cells requires both Ras-Raf-1- and Rap1-B-Raf-dependent pathways." in: Molecular and cellular biology, Vol. 21, Issue 8, pp. 2659-70, 2001 (PubMed).

Dhillon, Meikle, Yazici et al.: "Regulation of Raf-1 activation and signalling by dephosphorylation." in: The EMBO journal, Vol. 21, Issue 1-2, pp. 64-71, 2002 (PubMed).