Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 644-819) antibody

Antigen: Breast Cancer Anti-Estrogen Resistance 1 (BCAR1)

Synonyms: CAS, CAS1, CASS1, CRKAS, P130Cas, Cas, Crkas, AI385681, P130CAS, MGC68466, cas, p130cas, BCAR1, bcar1, fc17f11, MGC175192, wu:fc17f11

Binding Site: AA 644-819

Clonality: Monoclonal (21)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)

Catalog no.: ABIN967871

Additional Information

Alternative name: p130 Cas

Immunogen: Rat p130 [Cas]

Cross-Reactivity: Human, Chicken, Dog (Canine), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 21

Description: P47 [v-crk] is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-crk protein is a fusion product of the viral gag protein and a part of cellular crk that includes SH2 and SH3 domains. v-crk induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130 [Cas]. p130 [Cas] is tightly associated with v-crk via the SH2 domain of v-crk. Tyrosine phosphorylation of p130 [Cas] occurs in conjunction with cellular transformation in cells that express v-src or v-crk. This phosphorylation leads to a change in p130 [Cas] localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130 [Cas] also associates with v-src, it may be a v-src substrate. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 130 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of p130 [Cas] on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- p130 [Cas] antibody.

Immunofluorescence staining of human fibroblasts.

Publications

Product: Sakai, Iwamatsu, Hirano et al.: "A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner." in: The EMBO journal, Vol. 13, Issue 16, pp. 3748-56, 1994 (PubMed).

Kook, Shim, Choi et al.: "Caspase-mediated cleavage of p130cas in etoposide-induced apoptotic Rat-1 cells." in: Molecular biology of the cell, Vol. 11, Issue 3, pp. 929-39, 2000 (PubMed).

Derkinderen, Toutant, Kadaré et al.: "Dual role of Fyn in the regulation of FAK+6,7 by cannabinoids in hippocampus." in: The Journal of biological chemistry, Vol. 276, Issue 41, pp. 38289-96, 2001 (PubMed).

Hermanto, Zong, Li et al.: "RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2345-65, 2002 (PubMed).

Nagashima, Endo, Ogita et al.: "Adaptor protein Crk is required for ephrin-B1-induced membrane ruffling and focal complex assembly of human aortic endothelial cells." in: Molecular biology of the cell, Vol. 13, Issue 12, pp. 4231-42, 2002 (PubMed).