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Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 644-819) antibody

Details for Product No. ABIN967871, Supplier: Login to see
Antigen
  • MGC68466
  • cas
  • Crkas
  • p130cas
  • BCAR1
  • fc17f11
  • wu:fc17f11
  • zgc:175192
  • CAS
  • CAS1
  • CASS1
  • CRKAS
  • P130Cas
  • AI385681
  • Cas
  • P130CAS
Alternatives
anti-Human Breast Cancer Anti-Estrogen Resistance 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 644-819
27
24
13
11
6
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
162
78
74
6
4
2
1
1
Host
Mouse
126
31
10
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
5
4
4
4
4
3
3
3
3
3
3
2
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
116
51
50
35
28
25
13
11
5
3
1
1
1
1
Supplier
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Immunogen Rat p130 [Cas] aa. 644-819
Clone 21-p130[Cas]
Isotype IgG1
Cross-Reactivity Human, Chicken, Dog (Canine), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p130 Cas (BCAR1 Antibody Abstract)
Background P47 [v-crk] is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-crk protein is a fusion product of the viral gag protein and a part of cellular crk that includes SH2 and SH3 domains. v-crk induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130 [Cas]. p130 [Cas] is tightly associated with v-crk via the SH2 domain of v-crk. Tyrosine phosphorylation of p130 [Cas] occurs in conjunction with cellular transformation in cells that express v-src or v-crk. This phosphorylation leads to a change in p130 [Cas] localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130 [Cas] also associates with v-src, it may be a v-src substrate. This antibody is routinely tested by western blot analysis.
Molecular Weight 130 kDa
Pathways EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 644-819) antibody (ABIN967871) Western blot analysis of p130 [Cas] on a human endothelial cell lysate. Lane 1: 1:100...
Immunofluorescence (IF) image for anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 644-819) antibody (ABIN967871) Immunofluorescence staining of human fibroblasts.
Product cited in: Nagashima, Endo, Ogita et al.: "Adaptor protein Crk is required for ephrin-B1-induced membrane ruffling and focal complex assembly of human aortic endothelial cells." in: Molecular biology of the cell, Vol. 13, Issue 12, pp. 4231-42, 2002 (PubMed).

Hermanto, Zong, Li et al.: "RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2345-65, 2002 (PubMed).

Derkinderen, Toutant, Kadaré et al.: "Dual role of Fyn in the regulation of FAK+6,7 by cannabinoids in hippocampus." in: The Journal of biological chemistry, Vol. 276, Issue 41, pp. 38289-96, 2001 (PubMed).

Kook, Shim, Choi et al.: "Caspase-mediated cleavage of p130cas in etoposide-induced apoptotic Rat-1 cells." in: Molecular biology of the cell, Vol. 11, Issue 3, pp. 929-39, 2000 (PubMed).

Background publications Sakai, Iwamatsu, Hirano et al.: "A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner." in: The EMBO journal, Vol. 13, Issue 16, pp. 3748-56, 1994 (PubMed).