Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1025-1203) antibody

Antigen: Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)

Synonyms: NOS, INOS, NOS2A, HEP-NOS, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, eNOS, Nos-3, ecNOS, 2310065A03Rik, iNos, cNOS, EC-NOS, NOSIII, NOS3, NOS2, nos2, ECNOS

Binding Site: AA 1025-1203

Clonality: Monoclonal (3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967886

Additional Information

Alternative name: eNOS/NOS Type III

Immunogen: Human eNOS

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 3

Description: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca[2+] levels and enhance calmodulin binding. Neuronal NOS (mNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and both are regulated in a similar manner. The human forms exhibit 52% amino acid identity. However, they are distinct gene products of about 155 kDa (mNOS) and 140 kDA (eNOS). The eNOS gene was cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC). eNOS protein has a unique N-myristylation consensus sequence that may explain its membrane localization.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Molecular Weight: 140 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of eNOS/NOS Type III on human endothelial cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-eNOS/NOS Type III.

Immunofluorescent staining of Endothelial Cells

Publications

Product: Zhao, Dugas, Mathiot et al.: "B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity." in: Blood, Vol. 92, Issue 3, pp. 1031-43, 1998 (PubMed).

Laufs, Endres, Stagliano et al.: "Neuroprotection mediated by changes in the endothelial actin cytoskeleton." in: The Journal of clinical investigation, Vol. 106, Issue 1, pp. 15-24, 2000 (PubMed).

Cao, Yao, McCabe et al.: "Direct interaction between endothelial nitric-oxide synthase and dynamin-2. Implications for nitric-oxide synthase function." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 14249-56, 2001 (PubMed).

Thomas, Chen, Keaney: "Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6017-24, 2002 (PubMed).

Kincer, Uittenbogaard, Dressman et al.: "Hypercholesterolemia promotes a CD36-dependent and endothelial nitric-oxide synthase-mediated vascular dysfunction." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23525-33, 2002 (PubMed).