TIA1 Cytotoxic Granule-Associated RNA Binding Protein-Like 1 (TIAL1) (AA 161-365) antibody

Antigen: TIA1 Cytotoxic Granule-Associated RNA Binding Protein-Like 1 (TIAL1)

Binding Site: AA 161-365

Clonality: Monoclonal (6)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967917

Additional Information

Alternative name: TIAR

Immunogen: Human TIAR

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Frog

Format: Liquid

Isotype: IgG1

Clone: 6

Description: TIAR is an RNA-binding protein related to TIA-1. Both proteins are candidate effectors of apoptotic cell death and consist of three N-terminal RNA-recognition motifs (RRM) and a C-terminal protein-interaction domain (PID). Unlike TIA-1, which is localized in the granules of cytotoxic lymphocytes, TIAR is predominantly found in the nucleus of many different cell types. Two related isoforms (42 kDa and 50 kDa) of TIAR have been identified. It has been demonstrated that TIAR can trigger DNA fragmentation in permeabilized thymocytes, suggesting that it may be an effector of apoptotic cell death. During Fas-mediated apoptosis, TIAR shows a rapid translocation from the nucleus to the cytoplasm. This redistribution of TIAR appears to be a specific event and possibly a general feature of the apoptotic program.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 50 & 42 kDa

Comments:

Related Products: ABIN968555, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of TIAR on a mouse macrophage cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-TIAR antibody.

Immunofluorescence staining of human endothelial cells.

anti-TIA1 Cytotoxic Granule-Associated RNA Binding Protein-Like 1 (TIAL1) (AA 161-365) antibody (Image 3)

Publications

Product: Kawakami, Tian, Duan et al.: "Identification and functional characterization of a TIA-1-related nucleolysin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8681-5, 1992 (PubMed).

Taupin, Tian, Kedersha et al.: "The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 5, pp. 1629-33, 1995 (PubMed).