Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody

Antigen: Tripartite Motif Containing 25 (TRIM25)

Synonyms: EFP, Z147, RNF147, ZNF147, Zfp147, AA960166, AL022677, MGC55773, zgc:55773, MGC130751, TRIM25, Efp, Znf147, MGC72607

Binding Site: AA 32-210

Clonality: Monoclonal (2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN968044

Additional Information

Alternative name: EFP

Immunogen: Human EFP

Format: Liquid

Isotype: IgG1

Clone: 2

Description: Steriod hormones, unlike polypeptide hormones, diffuse inside the cell, bind cytoplasmic or nuclear receptors, and activate specific genes. Complete identification of such receptors would greatly increase the understanding of the celluar processes activated by steriods. EFP (Estrogen receptor Finger Protein) was identified as a result of its specific interaction with the estrogen receptor. EFP contains a zinc-finger domain, indicating that it is a DNA-binding protein. EFP transcription is regulated by estrogens and, in combination with the estrogen receptor, it may modulate the expression of estrogen-responsive genes. This antibody is routinely tested by western blot analysis.
Synonyms: Estrogen receptor Finger Protein

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 71 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of EFP on a human endothelial cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-human EFP antibody.

Immunofluorescence staining of human endothelial cells.

anti-Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody (Image 3)

Publications

Product: Inoue, Orimo, Hosoi et al.: "Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11117-21, 1994 (PubMed).