Gephyrin (GPHN) (AA 569-726) antibody

Antigen: Gephyrin (GPHN)

Synonyms: GPH, geph, GPHRYN, AI662856, BC027112, MGC38765, MGC150066, C230040D23, 5730552E08Rik, Geph, GPHN, gphn, MGC84626, GEPH, KIAA1385, gpgb1, DKFZp459M0825

Binding Site: AA 569-726

Clonality: Monoclonal (45)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968050

Additional Information

Alternative name: Gephyrin

Immunogen: Rat Gephyrin

Cross-Reactivity: Chicken, Human, Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 45

Description: The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 93 kDa

Comments:

Related Products: ABIN968546, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Gephyrin.

Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, 20X.

Publications

Product: Prior, Schmitt, Grenningloh et al.: "Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein." in: Neuron, Vol. 8, Issue 6, pp. 1161-70, 1992 (PubMed).

Meyer, Kirsch, Betz et al.: "Identification of a gephyrin binding motif on the glycine receptor beta subunit." in: Neuron, Vol. 15, Issue 3, pp. 563-72, 1995 (PubMed).

Feng, Tintrup, Kirsch et al.: "Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1321-4, 1998 (PubMed).

Kneussel, Haverkamp, Fuhrmann et al.: "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8594-9, 2000 (PubMed).