Gephyrin (GPHN) (AA 569-726) antibody
|Synonyms||GPH, geph, GPHRYN, AI662856, BC027112, MGC38765, MGC150066, C230040D23, 5730552E08Rik, Geph, GPHN, gphn, MGC84626, GEPH, KIAA1385, gpgb1, DKFZp459M0825|
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
|4 references available|
|Quantity||150 µg (250 µg/ml) (Variants)|
|Price||Product not available in this region.|
|Cross-Reactivity||Chicken, Human, Mouse (Murine)|
|Description||The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||93 kDa|
Related Products: ABIN968546, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Prior, Schmitt, Grenningloh et al.: "Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein." in: Neuron, Vol. 8, Issue 6, pp. 1161-70, 1992 (PubMed).
Meyer, Kirsch, Betz et al.: "Identification of a gephyrin binding motif on the glycine receptor beta subunit." in: Neuron, Vol. 15, Issue 3, pp. 563-72, 1995 (PubMed).
Feng, Tintrup, Kirsch et al.: "Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1321-4, 1998 (PubMed).
Kneussel, Haverkamp, Fuhrmann et al.: "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8594-9, 2000 (PubMed).
|Hosts||Rabbit (34), Mouse (17)|
|Reactivities||Human (48), Mouse (Murine) (24), Rat (Rattus) (23), Dog (Canine) (21), Cow (Bovine) (20), Horse (Equine) (18), Pig (Porcine) (18), Chicken (3), Xenopus laevis (2), Zebrafish (2), Cat (Feline) (1), Monkey (1)|
|Applications||Western Blotting (WB) (36), Immunofluorescence (IF) (23), Flow Cytometry (FACS) (18), ELISA (11), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Center (2), C-Term (1), Internal Region (1), Internal Region,AA 444-474 (1), N-Term (1)|