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Gephyrin antibody (GPHN) (AA 569-726)

Details for Product anti-GPHN Antibody No. ABIN968051, Supplier: Login to see New
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Antigen
Synonyms GPHN, gpgb1, DKFZp459M0825, GEPH, GPH, GPHRYN, HKPX1, 5730552E08Rik, AI662856, BC027112, C230040D23, geph, Geph
Epitope
AA 569-726
(16), (10), (10), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
(76), (23), (21), (4), (3), (2), (2), (2), (1), (1), (1)
Host
Mouse
(44), (31), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
This Gephyrin antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (1)
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(71), (40), (27), (23), (13), (7), (7), (2), (1)
Pubmed 4 references available
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Quantity 150 μg
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Immunogen Rat Gephyrin aa. 569-726
Clone 45-Gephyrin
Isotype IgG1
Cross-Reactivity Chicken, Human, Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Gephyrin (GPHN Antibody Abstract)
Background The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules. This antibody is routinely tested by western blot analysis.
Molecular Weight 93 kDa
Research Area Neurology
Pathways
Comment

Related Products: ABIN968546, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Gephyrin antibody (GPHN) (AA 569-726) (ABIN968051) Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Gephyrin antibody (GPHN) (AA 569-726) (ABIN968051) Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, ...
 image for anti-Gephyrin antibody (GPHN) (AA 569-726) (ABIN968051) anti-Gephyrin (GPHN) (AA 569-726) antibody (Image 3)
Product cited in: Kneussel, Haverkamp, Fuhrmann et al.: "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8594-9, 2000 (PubMed).

Feng, Tintrup, Kirsch et al.: "Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1321-4, 1998 (PubMed).

Meyer, Kirsch, Betz et al.: "Identification of a gephyrin binding motif on the glycine receptor beta subunit." in: Neuron, Vol. 15, Issue 3, pp. 563-72, 1995 (PubMed).

Prior, Schmitt, Grenningloh et al.: "Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein." in: Neuron, Vol. 8, Issue 6, pp. 1161-70, 1992 (PubMed).

Catalog No. ABIN968051
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