RAC1 antibody

Antigen: RAC1

Clonality: Monoclonal (102)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968089

Additional Information

Immunogen: Human Rac1 Protein

Cross-Reactivity: Rat (Rattus), Chicken, Dog (Canine), Mouse (Murine)

Format: Liquid

Isotype: IgG2b

Clone: 102

Description: Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, like many of its relatives, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. In addition, IQGAP1 has been identified as a GTPase activating protein specific for Rac1. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 21 kDa

Comments:

Related Products: ABIN967389, ABIN968545

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Immunology, Innate Immunity

Restrictions: For Research Use only

Images

Western blot analysis of Rac1 on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Rac1 antibody.

Immunofluorescent staining of human fibroblast cells.

anti-RAC1 antibody (Image 3)

Publications

Product: Didsbury, Weber, Bokoch et al.: "rac, a novel ras-related family of proteins that are botulinum toxin substrates." in: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16378-82, 1989 (PubMed).

Nobes, Hall: "Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia." in: Cell, Vol. 81, Issue 1, pp. 53-62, 1995 (PubMed).

Innocenti, Tenca, Frittoli et al.: "Mechanisms through which Sos-1 coordinates the activation of Ras and Rac." in: The Journal of cell biology, Vol. 156, Issue 1, pp. 125-36, 2002 (PubMed).

Doye, Mettouchi, Bossis et al.: "CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion." in: Cell, Vol. 111, Issue 4, pp. 553-64, 2002 (PubMed).

Habas, Dawid, He: "Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation." in: Genes & development, Vol. 17, Issue 2, pp. 295-309, 2003 (PubMed).