Protein Kinase N1 (PKN1) (AA 215-388) antibody
|Synonyms||DBK, PKN, PAK1, PRK1, PRKCL1, MGC46204, PKN-ALPHA, Pkn, Stk3, Prkcl1, F730027O18Rik, PKN1, MGC142408, MGC162290, PAK-1|
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
|4 references available|
|Quantity||50 µg (250 µg/ml) (Variants)|
|Price||Product not available in this region.|
|Cross-Reactivity||Dog (Canine), Rat (Rattus), Mouse (Murine)|
Members of the Protein Kinase C (PKC) family of homologous serine/threonine protein kinases are involved in a number of processes such as cell growth, cell differentiation, and cytokine secretion. PKCs are activated by Ca2+, phospholipids, diacylglycerol, phorbol esters, and proteolysis. PRK1 (PKC-Related Kinase 1, also named PKN) was originally identified in human hippocampus as a novel protein kinase with sequence homology to PKC. PRK1 contains 942 amino acids with an apparent molecular weight of 120 kDa. Although activated by limited proteolysis, PRK1 is not activated by Ca2+/diacylglycerol or phorbol esters. However, PRK1 is activated by phospholipids and arachidonic acid. PRK1 may regulate cytoskeletal changes since it binds to Rho-GTP and becomes phosphorylated in vivo, coincidentally with the formation of focal adhesions and stress fibers.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||120 kDa|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of PRK1 on Jurkat lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of PRK1. MCF7|
Palmer, Ridden, Parker: "Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family." in: European journal of biochemistry / FEBS, Vol. 227, Issue 1-2, pp. 344-51, 1995 (PubMed).
Mukai, Kitagawa, Shibata et al.: "Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis." in: Biochemical and biophysical research communications, Vol. 204, Issue 1, pp. 348-56, 1994 (PubMed).
Flynn, Mellor, Casamassima et al.: "Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11064-70, 2000 (PubMed).
Hughes, Larijani, Parker: "Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22974-9, 2002 (PubMed).
|Hosts||Rabbit (11), Mouse (1)|
|Reactivities||Human (13), Rat (Rattus) (2), Dog (Canine) (1), Mouse (Murine) (1)|
|Applications||Western Blotting (WB) (12), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (2), Dot Blot (DB) (1), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1)|
|Epitopes||C-Term (3), N-Term (3), AA 911-929 (1), AA 918-930 (1), pThr778 (1)|